کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9442969 1302612 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Entamoeba histolytica: Functional characterization of the −234 to −196 bp promoter region of the multidrug resistance EhPgp1 gene
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Entamoeba histolytica: Functional characterization of the −234 to −196 bp promoter region of the multidrug resistance EhPgp1 gene
چکیده انگلیسی
The multidrug resistance EhPgp1 gene is constitutively expressed in drug resistant trophozoites from Entamoeba histolytica. It has been demonstrated that two CCAAT/enhancer binding sites located in the EhPgp1 gene promoter control its transcriptional activation. However, functional assays of the 5′ end of its promoter showed that region from −234 to −196 bp (38 bp) is also important for the EhPgp1 gene transcription. Here, we demonstrated that in the 38 bp region putative cis-activator sequences are located. In silico analysis showed the presence of GATA1, Gal4, Nit-2, and C/EBP consensus sequences. Additionally, we identified three specific DNA-protein complexes, which were competed by a C/EBP, GATA1, and HOX oligonucleotides. Finally, we partially purified three proteins of 64.4, 56.7, and 27.4 kDa. Further investigations are currently in progress to determine the identity of these nuclear factors and how they are interacting with the EhPgp1 gene promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 110, Issue 3, July 2005, Pages 238-243
نویسندگان
, , , ,