کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9443001 1302616 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flow cytometric assessment of Leishmania spp metacyclic differentiation: Validation by morphological features and specific markers
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Flow cytometric assessment of Leishmania spp metacyclic differentiation: Validation by morphological features and specific markers
چکیده انگلیسی
Characterization of infective metacyclic promastigotes of Leishmania spp can be an essential step in several experimental protocols. Metacyclic forms of all Leishmania species display a typical morphology with short, narrow cell body, and an elongated flagellum. This feature suggests that metacyclics can be distinguished from procyclic forms by non-fluorimetric flow cytometric parameters thus enabling the follow-up of their appearance and acquisition of specific properties, during metacyclogenesis in in vitro cultures. Here we describe the flow cytometric parameters of stage-specific promastigotes of Leishmania major, Leishmania donovani, Leishmania amazonensis, and Leishmania braziliensis. Our findings were validated by optical microscopy morphology and specific procyclic labeling with FITC-peanut agglutinin. Furthermore, we show that parasite's distribution in the plot during differentiation in culture is not species specific and that the parasites displaying low forward-angle light scatter (FSClow) are three times more infective than the FSChigh ones. The method here described can be applied to the identification of metacyclics of different Leishmania spp within the whole stationary population.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 110, Issue 1, May 2005, Pages 39-47
نویسندگان
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