کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9464562 | 1314472 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intestinal metabolism of PAH: in vitro demonstration and study of its impact on PAH transfer through the intestinal epithelium
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کلمات کلیدی
CyPBDMPYRFCSPHEBAPEGFPAHDMSO - DMSOBenzo[a]pyrene - بنزو [a] پییرنDimethylsulfoxide - دیمتیل سولفواکسیدfetal calf serum - سرم گوساله جنینCaco-2 cells - سلول های Caco-2cytochrome - سیتوکرومepidermal growth factor - عامل رشد اپیدرمیMetabolism - متابولیسم Polycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایPAHs - هیدروکربن های آروماتیک چند حلقه ایPhenanthrene - پنانترنPyrene - پیرنه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Food would seem to be one of the main ways of animal and human contamination with polycyclic aromatic hydrocarbons (PAHs). In vivo studies suggest a transfer in intestinal epithelium by diffusion, which appears extensively governed by the physicochemical properties of PAHs, particularly lipophilicity. However, other mechanisms, such as metabolism, are considered to intervene. Our work aimed at testing in vitro intestinal metabolism and defining its impact on transepithelial transport of PAHs. Caco-2 cells were cultivated on permeable filters and incubated with 14C-labeled benzo[a]pyrene (BaP), pyrene (Pyr), and phenanthrene (Phe), which differ in their physicochemical properties. The results showed that the cells were able to metabolize the compounds. In basal media, Phe appeared to be the least hydroxylated molecule (45% after a 6-h exposure), followed by Pyr (65%) and finally BaP (96%). Inhibition of PAH metabolism showed a determinant effect on kinetics profiles. Transfer in the basal compartment of BaP, Pyr, and Phe radioactivities was, respectively, 26, 4, and 2 times lower with inhibitors, corroborating that intestinal metabolism of PAHs would have a positive impact on their transfer, an impact that increased with their lipophilicity. Furthermore, after a 6-h incubation, metabolites were also detected in apical medium. These findings suggested that intestinal metabolism might play a key role in intestinal barrier permeability and thus in the bioavailability of tested micropollutants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Research - Volume 98, Issue 1, May 2005, Pages 22-32
Journal: Environmental Research - Volume 98, Issue 1, May 2005, Pages 22-32
نویسندگان
Séverine Cavret, Cyril Feidt,