کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9487441 | 1330748 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel cyclodextrin glycosyltransferase from an alkalophilic Bacillus species: purification and characterization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cyclodextrin glycosyltransferase (E.C. 2.4.1.19) of alkalophilic Bacillus sp. 7-12 was purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sepharose CL-6B column chromatography. The enzyme thus obtained consisted of a single band that did not dissociate into subunits by SDS-polyacrylamide gel electrophoresis (PAGE). The molecular weight of the purified enzyme was determined to be 69,000 Da by SDS-PAGE. The enzyme was stable below 70 °C with an optimum activity at 60 °C, and was stable at a pH range of 6-10 with an optimum pH at 8.5. The enzyme activity was strongly inhibited by MgCl2, ZnCl2, CuSO4, Al2(SO4)3, CoCl2, AgNO3, FeSO4 and slightly inhibited by SnCl2 and MnCl2. CaCl2, KCl, EDTA and DTT had no influence on the enzyme activity. For cyclodextrin production, up to 34% conversion to cyclodextrins was obtained from 10% starch. The enzyme produced α-, β- and γ-cyclodextrins in the ratio of 0.26:1:0.86.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 38, Issue 3, April 2005, Pages 309-314
Journal: Food Research International - Volume 38, Issue 3, April 2005, Pages 309-314
نویسندگان
Xinzhi Cao, Zhengyu Jin, Xi Wang, Feng Chen,