کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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95915 | 160449 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous determination of creatinine and uric acid in urine by liquid chromatography–tandem mass spectrometry with polarity switching electrospray ionization Simultaneous determination of creatinine and uric acid in urine by liquid chromatography–tandem mass spectrometry with polarity switching electrospray ionization](/preview/png/95915.png)
A simple liquid chromatography–tandem mass spectrometric (LC–MS/MS) method was developed and validated to simultaneously determine creatinine (Cr) and uric acid (UA) levels as a confirmatory method for adulteration or dilution of urine. Centrifuged urine samples (10 μL) were diluted with 390 μL of distilled water. 30 μL of internal standard solution (Cr-d3, 5 μg/mL) and 10 μL of acetonitrile were added to 20 μL aliquots of diluted urine samples and filtered. The samples (1 μL) were introduced into LC–MS/MS with no further pretreatment. Cr and UA were separated on a multi-mode ODS column (Scherzo SM-C18, 75 mm × 2.0 mm I.D., 3 μm) and quantified by LC–MS/MS with polarity-switching electrospray ionization. Cr requires the positive-ion mode, whereas the negative-ion mode is required for the analysis of UA. The linear ranges were 1.0–300 mg/dL for Cr and 0.5–300 mg/dL for UA, with good determination coefficients (R2 ≥ 0.9988). The intra-day and inter-day precision of the analytes was within 13.0% and 14.4%, respectively. The intra-day and inter-day accuracy was −8.8 to 3.7% and −0.3 to 6.6%, respectively. The lower limits of detection (LLODs) were 0.3 mg/dL for Cr and 0.07 mg/dL for UA. The applicability of the developed method was examined by analyzing urine samples from suspected drug abusers (n = 46).
Journal: Forensic Science International - Volume 221, Issues 1–3, 10 September 2012, Pages 57–64