کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9607682 | 45147 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution](/preview/png/9607682.png)
چکیده انگلیسی
The development and application of an immobilized horseradish peroxidase (HRP) system with porous aminopropyl glass (APG) beads are reported. The optimal condition for the immobilization of HRP on APG with glutaraladhyde, which leads to a specific activity of 50.7 U/g APG, was identified. Upon immobilization, the operation pH range was broadened downward by one pH unit from 7.5-9.5 to 6.5-9.5 and optimal operation temperature was increased by at least 10 °C. Thermostability of HRP was also improved by approximately 20% upon immobilization. The application of such HRP immobilized APG for the removal of p-chlorophenol from water was investigated. An HPLC method for the quantification of p-chlorophenol was developed to substitute for spectrophotometric methods that are subject to interferences from proteins and other UV-absrobing compounds in the reaction mixtures. The polymerization of p-chlorophenol into insoluble precipitate was completed within 3 h after the initiation of reaction with the addition of hydrogen peroxide with a maximal removal efficiency of 25%, observed at pH 7.5. The addition of poly(ethylene glycol) significantly enhanced the removal efficiency presumably via the formation of a protective shield in the vicinity of the active site of HRP from the free radicals formed during polymerization and can thus reduce the amount of enzymes needed to achieve the desired removal efficiency. The reusability of the HRP immobilized APG was also demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1167-1174
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1167-1174
نویسندگان
Yi-Chen Lai, Sung-Chyr Lin,