کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9607715 | 45147 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of nutrient, pH and dissolved oxygen on the production of Metarhizium flavoviride Mf189 blastospores in submerged batch culture
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The influence of different parameters on the sporulation of Metarhizium flavoviride was studied during submerged cultures in shake flasks and in 5 l bioreactors. The screening in shake flasks of several carbon and nitrogen sources allowed the definition of an optimal medium, based on sucrose and brewer's yeast with a C/N ratio of 1.6. With this medium, a production of 5.4 à 108 blastospores per ml (Bspores mlâ1) was obtained after 169 h of cultivation. The influence of pH and pO2 was independently studied in 5 l working volume bioreactors using the optimal medium. The best production was obtained with pH and pO2 regulated respectively to 7 and 100%. Finally, when the culture was grown under optimized conditions, the blastospores concentration increased 16-fold, with 1.1 à 109 Bspores mlâ1 obtained after 144 h cultivation. This represents a gain of productivity of about 4.8 times.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1425-1431
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1425-1431
نویسندگان
N. Issaly, H. Chauveau, F. Aglevor, J. Fargues, A. Durand,