کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9607720 | 45147 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and characterization of a novel glycoprotein from wheat germ water-soluble extracts
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel glycoprotein, referred to as WGGP, was isolated from wheat germ water-soluble extracts, and purified by ion-exchange and gel filtration column chromatography. The homogeneity of WGGP was demonstrated by gel filtration on Sepharose CL-6B. The glycoprotein contains 56.4% of protein, rich in glutamic acid, asparagic acid, alanine, glycine, valine and leucine. Mannose accounted for 94% of the neutral sugar moiety, and traces of arabinose, xylose, glucose and galactose were found in WGGP. The molecular weight was estimated to be about 40,000 on SDS-PAGE. The existence of O-glycosidic linkage in WGGP was demonstrated with a β-elimination reaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1469-1474
Journal: Process Biochemistry - Volume 40, Issues 3â4, March 2005, Pages 1469-1474
نویسندگان
Kexue Zhu, Huiming Zhou,