کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9622563 | 160559 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening and confirmatory method for benzodiazepines and hypnotics in oral fluid by LC-MS/MS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Screening and confirmatory method for benzodiazepines and hypnotics in oral fluid by LC-MS/MS Screening and confirmatory method for benzodiazepines and hypnotics in oral fluid by LC-MS/MS](/preview/png/9622563.png)
چکیده انگلیسی
A procedure is presented for the screening of 17 benzodiazepines and hypnotics in oral fluid after collection with the Intercept® device by LC-MS/MS (alprazolam, 7-aminoclonazepam, 7-aminoflunitrazepam, bromazepam, clobazam, diazepam, lorazepam, lormetazepam, midazolam, nordiazepam, oxazepam, temazepam, tetrazepam, triazolam, zaleplon, zopiclone and zolpidem). The method involves extraction of 0.5Â mL of oral fluid (previously stored in the Intercept blue buffer) treated with 0.5Â mL of phosphate buffer (pH 8.4) in the presence of 5Â ng diazepam-d5 used as internal standard, with 3Â mL of diethyl ether/methylene chloride (50/50) and separation using liquid chromatography-tandem mass spectrometry. The limits of quantification for all benzodiazepines and hypnotics range from 0.1 to 0.2Â ng/mL. Linearity is observed from the limit of quantification of each compound to 20Â ng/mL (r2Â >Â 0.99). Coefficients of variation at 2Â ng/mL, measured on 6 points range from 4 to 8% for all drugs, except zopiclone (34%). Extraction recovery, measured at the same concentration was higher than 90%. Ion suppression was evaluated for each compound and was lower than 10% for all drugs except zopiclone (93%). These results were found suitable to screen for 17 benzodiazepines in oral fluid and detect them at very low concentrations, making this method suitable for monitoring subjects under the influence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 150, Issues 2â3, 10 June 2005, Pages 213-220
Journal: Forensic Science International - Volume 150, Issues 2â3, 10 June 2005, Pages 213-220
نویسندگان
Pascal Kintz, Marion Villain, Marta Concheiro, Vincent Cirimele,