Quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in a kratom (Mitragyna speciosa Korth.) cocktail using high-performance liquid chromatography

A simple HPLC technique for determining mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in ‘kratom cocktail’ was developed. The analytical method for mitragynine, codeine and caffeine used an Eclipse XDB-C8 column. A Lichrospher CN column was using for analysing chlorpheniramine and phenylephrine. The correlation coefficient of each standard was between 0.9957 and 0.9993. The precision of the methods were between 0.700 and 7.108% RSD. The concentration of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in ‘kratom cocktail’ was 90.021, 234.174, 73.986, 7.053 and 1.486 mg/L, respectively.