کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9678420 | 1454326 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nanorheology measurement on single circularly permuted green fluorescent protein molecule
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Nanorheology measurement on single circularly permuted green fluorescent protein molecule Nanorheology measurement on single circularly permuted green fluorescent protein molecule](/preview/png/9678420.png)
چکیده انگلیسی
The force measurement mode of an atomic force microscope (AFM) has enabled us to measure the mechanical properties of biological materials at the single molecular level. In a conventional quasi-static force measurement on a single circularly permuted green fluorescent protein (cpGFP), we could unfold it by unraveling several sub-domains in a distinct sawtooth pattern at a slow stretching speed. In order to elucidate more detailed conformational changes at each extension length, we further measured dynamic relax-stress response of cpGFP molecules. In this measurement, several cycles of sinusoidal movement were applied to the sample during the stretching process. We found the protein molecule showed in-phase response to the sinusoidal input in most case of measurements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 40, Issues 3â4, 25 February 2005, Pages 183-187
Journal: Colloids and Surfaces B: Biointerfaces - Volume 40, Issues 3â4, 25 February 2005, Pages 183-187
نویسندگان
Tong Wang, Yasuhiro Sakai, Ken Nakajima, Atsushi Miyawaki, Kohzo Ito, Masahiko Hara,