کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9684982 | 1456219 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of spacer arm on penicillin G acylase purification using immobilized metal affinity membranes
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of spacer arm on penicillin G acylase purification using immobilized metal affinity membranes Effects of spacer arm on penicillin G acylase purification using immobilized metal affinity membranes](/preview/png/9684982.png)
چکیده انگلیسی
For studying penicillin G acylase (PGA) purification efficiency, various chain lengths of diamine (n = 2-10) were applied as spacer arm to prepare the regenerated cellulose-based immobilized metal affinity membrane (IMAM). The effects of spacer arm length on water content of the IMAM, immobilized metal ion capacity, and batch PGA adsorption were investigated. The use of 1,10-diaminodecane led to the smallest water content of membrane and the maximum capacity of Cu2+ (62.06 μmol/disc), whereas the optimal PGA adsorption capacity (2.6 IU/disc) was achieved with 1,8-diaminooctane. When applying the IMAMs with the spacer arm of 1,8-diaminooctane to the flow chromatographic experiments, a performance of 2.73-fold purification and 70.27% recovery was obtained. The employment of diamine spacer arm on the preparation of IMAMs resulted in higher PGA adsorption amount but less purification efficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 251, Issues 1â2, 1 April 2005, Pages 201-207
Journal: Journal of Membrane Science - Volume 251, Issues 1â2, 1 April 2005, Pages 201-207
نویسندگان
Yung-Chuan Liu, Shing-Yi Suen, Cheng-Weng Huang, Chih-Chiang ChangChien,