کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9756931 | 1495289 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique](/preview/png/9756931.png)
چکیده انگلیسی
At pH 9.75, the resonance light scattering (RLS) intensity of OA-Eu3+ system is greatly enhanced by nucleic acid. Based on this phenomenon, a new quantitative method for nucleic acid in aqueous solution has been developed. Under the optimum condition, the enhanced RLS is proportional to the concentration of nucleic acid in the range of 1.0Â ÃÂ 10â9 to 1.0Â ÃÂ 10â6Â g/ml for herring sperm DNA, 8.0Â ÃÂ 10â10 to 1.0Â ÃÂ 10â6Â g/ml for calf thymus DNA and 1.0Â ÃÂ 10â9 to 1.0Â ÃÂ 10â6Â g/ml for yeast RNA, and their detection limits are 0.020, 0.011 and 0.010Â ng/ml, respectively. Synthetic samples and actual samples were satisfactorily determined. In addition, the interaction mechanism between nucleic acid and OA-Eu3+ is also investigated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 62, Issues 4â5, December 2005, Pages 896-901
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 62, Issues 4â5, December 2005, Pages 896-901
نویسندگان
Xia Wu, Shuna Sun, Jinghe Yang, Minqin Wang, Liyan Liu, Changying Guo,