کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9758952 1496933 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly
چکیده انگلیسی
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 66, Issues 1–2, April 2005, Pages 139-143
نویسندگان
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