کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9811 648 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses
چکیده انگلیسی

Highly ordered MCM-41-type mesoporous silica nanoparticles (MSNs) with particle sizes of 150 ± 20 nm were prepared and PEGylated by covalently grafting PEGxk chains of different molecular weights (x = 4, 6, 10, 20) and chain densities (0.05 wt%–3.75 wt%) on the outer surface. The influence of molecular weights and chain densities of PEGxk on the nonspecific binding of PEGylated MSNs to human serum protein (HSA) was investigated. The results revealed that the optimal molecular weights should be not less than 10 k, and the corresponding optimal chain densities for PEG10k–MSNs and PEG20k–MSNs were 0.75 wt% and 0.075 wt%, respectively, and the resultant minimum HSA adsorbance (2.5%) on PEGxk–MSNs was far less than that on MSNs (18.7%) without PEGylation. Under the optimal conditions for the minimum HSA adsorbance, the phagocytosis of human THP-1 monocytic leukemia cell line-derived macrophages (THP-1 macrophages) and the hemolysis of human red blood cells (HRBCs) were investigated with MSNs and PEGylated MSNs. A minimum THP-1 phagocytosis percentage (0.1%) and a very low HRBCs hemolysis percentage (0.9%) of PEG10k–MSNs were obtained, which were much lower than those (8.6% and 14.2%, respectively) of MSNs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 6, February 2010, Pages 1085–1092
نویسندگان
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