کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9890858 | 1540336 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of the bioactive compound 5,7,4â²-trihydroxy-6,3â²,5â²-trimethoxyflavone to human serum albumin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
5,7,4â²-trihydroxy-6,3â²,5â²-trimethoxyflavone is one of the bioactive components isolated from Artemisia plants possessing antitumor therapeutic activities. In this paper, its binding properties and binding sites located on human serum albumin (HSA) have been studied using UV absorption spectroscopy, fluorescence spectroscopy and Fourier transform infrared (FT-IR) spectra. The results of fluorescence titration revealed that 5,7,4â²-trihydroxy-6,3â²,5â²-trimethoxyflavone could strongly quench the intrinsic fluorescence of HSA by static quenching and there was only one class of binding sites on HSA for this drug. The binding constants at four different temperatures (289, 298, 310, and 318 K) were 1.93, 1.56, 1.22, and 0.93 Ã 105 L molâ1, respectively. The FT-IR spectra evidence showed that the protein secondary structure changed with reduction of α-helices about 27.6% at the drug to protein molar ratio of 3. The thermodynamic functions standard enthalpy change (ÎH0) and standard entropy change (ÎS0) for the reaction were calculated to be â18.70 kJ molâ1 and 36.62 J molâ1 Kâ1 according to the van't Hoff equation. These results and the molecular modeling study suggested that hydrophobic interaction was the predominant intermolecular force stabilizing the complex, and 5,7,4â²-trihydroxy-6,3â²,5â²-trimethoxyflavone could bind to the site I of HSA (the Warfarin Binding site).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 37, Issues 1â2, 30 October 2005, Pages 85-91
Journal: International Journal of Biological Macromolecules - Volume 37, Issues 1â2, 30 October 2005, Pages 85-91
نویسندگان
Jianghong Tang, Weiping Wang, Feng Luan, Xingguo Chen,