کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9901413 1545419 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-invasive pulmonary aerosol delivery in mice by the endotracheal route
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Non-invasive pulmonary aerosol delivery in mice by the endotracheal route
چکیده انگلیسی
In this report we present in detail a non-invasive pulmonary application method that can be a useful tool in studying drug and vaccine delivery to the lower airways. In this method the formulation is sprayed directly into the lungs of mice via the endotracheal route using a MicroSprayer™ aerolizer. Mean droplet size produced was 8 μm, appropriate for deposition in the large airways. Endotracheal application of suspension of fluorescent nanospheres, 200 nm in size, by this method resulted in nanoparticle deposition in the smaller airways (bronchi and bronchioles). Mice showed full recovery one day after administration of 50 μl of formulation. Furthermore, no mortality was observed as a result of the technique. We conclude that this endotracheal application is a useful tool for studying pulmonary drug delivery in mice. The technique is especially useful for the pulmonary application of vaccines, since it enables multiple administrations without a need for analgesics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 61, Issue 3, October 2005, Pages 214-218
نویسندگان
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