کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902237 | 1545795 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification and characterization of myosin peptide-specific CD4+ T cells in autoimmune myocarditis
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کلمات کلیدی
SFCAPCELISPOTEAM - POMspot forming cell - سلول تشکیل دهنده نقطهDendritic cell - سلول دندریتیکT helper cells - سلول های کمکیantigen presenting cell - سلولهای پردازنده آنتیژنCytokines - سیتوکین هاAutoimmune myocarditis - میوکاردیت اتوایمونexperimental autoimmune myocarditis - میوکاردیت خود ایمنی تجربی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantification and characterization of myosin peptide-specific CD4+ T cells in autoimmune myocarditis Quantification and characterization of myosin peptide-specific CD4+ T cells in autoimmune myocarditis](/preview/png/9902237.png)
چکیده انگلیسی
Characterization of autoantigen-specific CD4+ T cells at the single cell level is crucial for understanding the immunopathological mechanisms underlying autoimmune diseases. Cardiac myosin heavy chain (myhca) is the major autoantigen associated with autoimmune myocarditis both in humans and in experimental autoimmune myocarditis (EAM) in mice. In the current study, we evaluated two methods for the enumeration and phenotypic characterization of myhca-specific CD4+ T cells during the course of EAM. Both enzyme-linked immunospot (ELISPOT) and cytokine flow cytometry (CFC) assays were suitable for the detection and characterization of myhca-specific Th cells during acute myocardial inflammation and the late healing phase of the disease. Cytokine production of myhca-specific Th cells was restricted to interferon-γ (IFNγ). Only trace amounts of the Th2 cytokines IL-4 and IL-5 could be detected. Concomitant surface marker analysis in the CFC assay revealed the prototypical effector phenotype of myhca-specific Th1 cells during the acute phase of the disease. Taken together, the combination of both methods appears to be most appropriate for a comprehensive ex vivo single cell analysis of Th cells in heart-specific autoimmune disorders.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 304, Issues 1â2, September 2005, Pages 117-125
Journal: Journal of Immunological Methods - Volume 304, Issues 1â2, September 2005, Pages 117-125
نویسندگان
Reinhard Maier, Simone Miller, Michael Kurrer, Philippe Krebs, Rita de Giuli, Marcel Kremer, Elke Scandella, Burkhard Ludewig,