کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902292 | 1545798 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cardiotrophin-like cytokine labelling using Bir A biotin ligase: A sensitive tool to study receptor expression by immune and non-immune cells
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کلمات کلیدی
IRESLIFRβCNTFRαCNTFRCLFAPCCNTFCLCFITCallophycocyanin - آلوفوکسیانینHuman - انسانReceptor expression - بیان گیرندهinternal ribosome entry site - سایت ورودی ریبوزوم داخلیcardiotrophin-like cytokine - سدیم کورتئوتروفین مانندCytokine - سیتوکینciliary neurotrophic factor - فاکتور نوروتروفیک ciliaryfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMouse - موش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We produced the cardiotrophin-like cytokine/cytokine-like factor-1 complex tagged with a Bir A biotin ligase AviTag peptide substrate. This cytokine could be efficiently biotinylated in vitro with Bir A. It was subsequently validated as a sensitive tool for ciliary neurotrophic factor receptor detection by flow cytometry and for magnetic-activated cell sorting. It was also shown to allow the detection of a specific receptor by activated B cells. Whereas binding to cells expressing the ciliary neurotrophic factor receptor could be prevented by competition with ciliary neurotrophic factor, binding to B cells was not. The biotinylated cardiotrophin-like cytokine/cytokine-like factor-1 complex therefore represents a new reagent to study ciliary neurotrophic factor and cardiotrophin-like cytokine receptor expression and for the identification of the putative cardiotrophin-like cytokine B cell receptor. It further validates the use of biotin ligase catalysed biotinylation for the detection of cytokine receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 301, Issues 1â2, June 2005, Pages 53-65
Journal: Journal of Immunological Methods - Volume 301, Issues 1â2, June 2005, Pages 53-65
نویسندگان
Isabelle Cognet, Florence Guilhot, Mélanie Gabriac, Sylvie Chevalier, Younes Chouikh, Alexandra Herman-Bert, Angélique Guay-Giroux, Sophie Corneau, Giovanni Magistrelli, Greg C. Elson, Hugues Gascan, Jean-François Gauchat,