کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902367 | 1545802 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of immune-complexed 9-O-acetylated sialoglycoconjugates in the sera of patients with pediatric acute lymphoblastic leukemia
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کلمات کلیدی
GalNAcDABBSMHRPFITCABTSTBS3,3′-diaminobenzidine tetrahydrochloride - 3،3'-diaminobenzidine tetrahydrochlorideBSA - BSAN-Acetyl galactosamine - N-استیل گالاکتوزامینbovine serum albumin - آلبومین سرم گاوTris-buffered saline - تریس بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتAcute lymphoblastic leukemia - لوسمی لنفوبلاستیک حادBovine submaxillary mucin - موکین submaxillary گاوALL - همهHorseradish peroxidase - پراکسیداز هوررادیشpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Although childhood acute lymphoblastic leukemia (ALL) is highly responsive to chemotherapy, reliable techniques are needed to determine treatment outcome. Over expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on lymphoblasts and concomitant anti-9-OAcSGs was found to have a diagnostic and prognostic potential. However, the presence of circulatory immune-complexed antigens remains unknown. The present study was aimed to evaluate whether immune-complexed 9-OAcSGs can be harnessed for better disease management. Immune-complexed antigens were evaluated in ALL sera (n=262) by a Dot-blot using a 9-OAcSAα2-6GalNAc-specific lectin, Achatinin-H. Using three serum samples, the inter- and intra-assay imprecision was evaluated as 11-13% and 7-11%, respectively. The recovery of spiked 9-OAcSGs was 84.2-95.4%. The central 95% reference interval for immune-complexed 9-OAcSGs in normal human sera (NHS, n=144) was 2.9-3.4 μg/ml irrespective of sex and age. At disease presentation, the immune-complexed 9-OAcSGs were fivefold higher than NHS, decreased with remission induction and importantly, reappeared with clinical relapse. Sera from patients with other hematological disorders (n=86) showed negligible levels. The Dot-blot demonstrated the potential application of immune-complexed antigen as a disease-specific marker and its efficacy as a sensitive and specific method that could serve as an economical yet effective index for monitoring disease status.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 13-26
Journal: Journal of Immunological Methods - Volume 297, Issues 1â2, February 2005, Pages 13-26
نویسندگان
Suman Bandyopadhyay, Kankana Mukherjee, Mitali Chatterjee, Dilip Kumar Bhattacharya, Chitra Mandal,