کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9905084 | 1546514 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of paired primary lung and lymph node tumor cells: A model of metastatic potential by multiple genetic programs
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
real-time quantitative RT-PCR - RT-PCR کمی زمان واقعیRNA isolation - جداسازی RNAHierarchical clustering - خوشه بندی سلسله مراتبیMicroarray - ریزآرایهNSCLC - سرطان ریوی غیر سلول کوچکlaser capture microdissection - لیزر ضبط میکرو دیسکسیونMetastasis - متاستاز Model - مدلMetastatic potential - پتانسیل متاستاتیکExpression profiling - پروفایل بیانnon-small-cell lung carcinoma - کارسینوم ریه غیر سلولی کوچک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background: The current paradigm of metastasis proposes that rare cells within primary tumors acquire metastatic capability via sequential mutations, suggesting that metastases are genetically dissimilar from their primary tumors. We tested this hypothesis by examining the molecular differences, if any, between primary tumor cells and matched lymph node metastatic cells in human non-small-cell lung carcinoma specimens. Methods: We performed transcriptional profiling studies on malignant cells from 11 pairs of stage III tumors and their tumor-positive lymph nodes using multiple, complementary analytic techniques. To confirm the overall validity of microarray data, we used real-time polymerase chain reaction. Results: The molecular signature of nodal metastasis was a composite of two paradoxical, but not mutually exclusive, expression patterns: metastatic cells are: (1) different from their primary tumor cells based on a few genes and (2) genetically similar, overall, to their primary tumor cells. Consequently, we found a 27-gene subset sufficient to differentiate nodal metastatic cells from primary tumor cells. Conclusions: Thus, we concluded that a more accurate model of metastatic potential is based on a global primary tumor expression pattern along with the appearance of distinct metastatic variants. The 27-gene signature differentiating primary tumors from their metastatic cells may define non-small-cell lung carcinoma nodal metastatic potential.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 509-517
Journal: Cancer Detection and Prevention - Volume 29, Issue 6, 2005, Pages 509-517
نویسندگان
Chuong D. MD, Tenner J. MD, Sean C. BS, Sherif H. MD, PhD, Robert A. MD, Michael A. MD,