کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9905898 | 1547308 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preoperative detection of laryngeal cancer in mucosal swabs by slide-based cytometry
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
The aim of our study was to evaluate slide-based cytometry in screening for laryngeal cancer using swabs as a minimally invasive approach. Laser scanning cytometry (LSC) was used for the multiparametric analysis of cells stained for cytokeratin and DNA to determine the DNA-index (DI) of the tumour cells. Histograms with DIÂ <Â 0.95, 1.05Â <Â DIÂ <Â 1.9, and 2.1Â <Â DI were defined as DNA aneuploid. After subsequent haematoxylin-eosin (HE)-staining, single cells were re-localised and an analysis by conventional cytology was performed. Additionally, routine histopathology of parallel biopsies was obtained in all cases. Fifty one swabs from 49 lesions were analysed. Seven and 17 swabs, were classified as insufficient for LSC and cytology, respectively. One and two benign lesions, were misclassified as malignant, respectively. Out of 34 malignant lesions, LSC detected 25 and cytology 14. LSC was superior to cytology in all of the statistical parameters tested. This pilot study demonstrates the validity of LSC for the preoperative detection of malignancy in laryngeal tumours using swabs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cancer - Volume 41, Issue 3, February 2005, Pages 445-452
Journal: European Journal of Cancer - Volume 41, Issue 3, February 2005, Pages 445-452
نویسندگان
Andreas O.H. Gerstner, Alexander Thiele, Attila Tárnok, Julia Machlitt, Jens Oeken, Andrea Tannapfel Andrea Tannapfel, Anette Weber, Friedrich Bootz,