کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9911047 1549056 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of target cells for Cholecystokinin in the porcine pancreas
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Determination of target cells for Cholecystokinin in the porcine pancreas
چکیده انگلیسی
Therefore, in this study we have determined the cell-type specific localization of CCK and its high affinity CCKA-receptor in islet cells using immunohistochemical techniques. Serial sectioning followed by double-immunostaining of methanol/acetic acid-fixed, paraffin-embedded pancreatic tissues were performed with antibodies against CCK, CCKA-receptor, glucagon and somatostatin. To determine whether CCK specific mRNA is locally expressed, total RNA was isolated, transcribed to cDNA and analysed with specific primer for CCK gene expression. Our results clearly show that CCK and the CCKA-receptor coexist in glucagon - but not in somatostatin-producing cells. Moreover our RT-PCR experiments demonstrate that there is no local gene expression of CCK in the porcine pancreas. Our results provide evidence that, in the porcine species, blood-borne CCK binds specifically to the CCKA-receptor and may thereby modulate the glucose homeostasis via a direct action on A-cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 187, Issue 3, 4 July 2005, Pages 209-214
نویسندگان
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