Bioinformatics analysis of a novel Glutaredoxin gene segment from a hot spring metagenomic DNA library

The present work describes the sequence, phylogenetic and structure analysis of a clone with insert size of 820 nucleotides obtained from the metagenomic library prepared from a hot spring located in the state of Uttarakhand, India. Open Reading Frame analysis revealed a coding sequence of 420 nucleotides. This region encodes a protein of 139 amino acids which depicts homologous relationship with glutaredoxin of Thermus sp. Multiple sequence alignment shows the classic glutaredoxin domain with a redox active CXXC motif, in the form of catalytic motif CLYC, suggesting this to be a novel glutaredoxin gene.