دانلود مقالات ISI درباره Bmgy + ترجمه فارسی

Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H

Keywords: Molecular cloning; Deglycosylation; Enzymatic assay; TLC; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; PCR; polymerase chain reaction; LB; lysogeny broth medium; MM; minimal medium; PBS; phosphate buffered saline; SDS-PA

دانلود رایگان متن کامل مقاله ISI 27 صفحه سال انتشار : 2019 سفارش ترجمه

Efficient production of human goose-type lysozyme 2 in the methylotrophic yeast Pichia pastoris

Keywords: hLysG2; human goose-type lysozyme 2; AOX1; alcoholoxidase 1; MD; minimal dextrose; YPD; yeast extract peptone dextrose; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; PCR; polymerase chain reaction; RT-PCR; real-time fluor

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2018 سفارش ترجمه

A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization

Keywords: Pichia pastoris / Komagataella phaffi; Broadly neutralizing antibody; VRC01; Signal peptide; 2A sequence; AA; α-amylase; α-MF; α-mating factor; αK; α-mating factor K; αKS; α-mating factor KS; αT; α-mating factor T; AOX1; alcohol oxidase 1; AP; al

دانلود رایگان متن کامل مقاله ISI 27 صفحه سال انتشار : 2018 سفارش ترجمه

Expression and characterization of the antimicrobial peptide ABP-dHC-cecropin A in the methylotrophic yeast Pichia pastoris

Keywords: Antimicrobial peptide; ABP-dHC-cecropin A; Antibacterial; Antifungal; pPICZαA; P. pastoris; ABP-dHC-cecropin A; antibacterial peptide drury (Hyphantriacunea) Cecropin A; AOX; alcohol oxidase; BMGY; buffered minimal glycerol-complex medium; MDH; minimal

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2017 سفارش ترجمه

Cloning and expression of the recombinant crustacean hyperglycemic hormone isoform B2 (rCHH-B2) and its effects on the metabolism and osmoregulation of the Pacific white shrimp Litopenaeus vannamei

Keywords: BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; EDTA; ethylenediaminetetraacetic acid; MES; 2-(N-morpholino) ethanesulfonic acid; PAM; peptidylglycine α-amidating monooxygenase; PBS; phosphate saline buffer; PCR; polymeras

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2017 سفارش ترجمه

Construction, expression and characterization of a fusion protein HBscFv-IFNγ in Komagatella (Pichia) pastoris X33

Keywords: AOX1; alcohol oxidase 1; BMGY; buffered minimal glycerol complex medium; BMMY; buffered minimal methanol-complex medium; BSA; bovine serum albumin; CB; Coomassie blue staining; ELISA; enzyme-linked immunosorbent assay; HBsAg; hepatitis B surface antigen;

دانلود رایگان متن کامل مقاله ISI 29 صفحه سال انتشار : 2017 سفارش ترجمه

Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris

Keywords: ABTS; 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt; BSM; basal salts medium; BMGY; buffered minimal glycerol yeast medium; BSA; bovine serum albumin; Ca-TBS; calcium-supplemented tris-buffered saline; DCW; dry cell weight; ELI

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2016 سفارش ترجمه

High-level expression of Proteinase K from Tritirachium album Limber in Pichia pastoris using multi-copy expression strains

Keywords: Proteinase K; Pichia pastoris; Multi-copy expression; qPCR; quantitative polymerase chain reaction; SDS; sodium dodecyl sulfate; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; MD; minimal dextrose medium; SDS-PAGE; sodium

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2016 سفارش ترجمه

Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris

Keywords: ABTS; 2,2′ azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt; BSM; basal salts medium; BMD1%; buffered minimal dextrose medium; BMG1%; buffered minimal glycerol medium; BMGY; buffered minimal glycerol yeast medium; BMM; buffered minimal m

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2016 سفارش ترجمه

Binding studies using Pichia pastoris expressed human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins

Keywords: Human AHR; Human ARNT; Pichia expression; Thermolysin; GA-hAHR; GeneArt optimized human aryl hydrocarbon receptor; WT-hAHR; wild type human aryl hydrocarbon receptor; WT-mAHR; wild type mouse aryl hydrocarbon receptor; GA-hARNT; GeneArt optimized human ar

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2016 سفارش ترجمه

Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis)

Keywords: (E, E)-farnesol; Heterologous expression; Moso bamboo; (E)-nerolidol; Pichia pastoris; Sesquiterpene; FPP; farnesyl pyrophosphate; YPD; yeast extract-peptone-dextrose; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; MM;

دانلود رایگان متن کامل مقاله ISI 25 صفحه سال انتشار : 2016 سفارش ترجمه

Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring

Keywords: PCR; polymerase chain reaction; BMGY; buffered glycerol complex medium; e-pPCR; end-point PCR; HCD; high cell density; qPCR; quantitative PCR; YPD; yeast extract peptone dextrose; SF; shake flask; wcw; wet cell weight; WCB; working cell bank; LRE; linear

دانلود رایگان متن کامل مقاله ISI 52 صفحه سال انتشار : 2016 سفارش ترجمه

Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter

Keywords: C12E8; octaethylene glycol monododecyl ether; MeAIB; (α-methylamino)isobutyric acid; BCH; 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid; YPDS; yeast extract peptone dextrose sorbitol; BMGY; buffered glycerol-complex medium; DOC; Na-deoxycholate; NP-40

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2015 سفارش ترجمه

Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

Keywords: P. pastoris; Pichia pastoris; L. vannamei (Lv); Litopenaeus vannamei; V. alginolyticus; Vibrio alginolyticus; B. subtilis; Bacillus subtilis; S. aureus; Staphylococcus aureus; A. veronii; Aeromonas veronii; BSA; bovine serum albumin; YPD; yeast extract pe

دانلود رایگان متن کامل مقاله ISI 16 صفحه سال انتشار : 2015 سفارش ترجمه

Production optimization and expression of pectin releasing enzyme from Aspergillus oryzae PO

Keywords: PPase; protopectinase; RSM; response surface methodology; BBD; Box-Behnken Design; PGase; polygalacturonase; PGA; polygalacturonic acid; MD; minimal dextrose medium; BMGY; buffered dextrose-complex medium; BMMY; buffered methanol-complex medium; Aspergi

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function

Keywords: PNPLA3; patatin-like phospholipase domain containing 3; ADPN; adiponutrin; Sf9; Spodoptera frugiperda 9; LPAAT; lysophosphatidic acid acyl transferase; YPDS; Yeast Extract Peptone Dextrose plus Sorbitol; BMGY; buffered glycerol-complex medium; BMMY; buffe

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Group 5 LEA protein, ZmLEA5C, enhance tolerance to osmotic and low temperature stresses in transgenic tobacco and yeast

Keywords: Low temperature stress; Osmotic stress; Phosphorylation; ZmLEA5C; ABA; abscisic acid; GFP; green fluorescent protein; LEA; late embryogenesis abundant; ORF; open reading frame; qRT-PCR; quantitative real-time reverse transcription-PCR; JA; jasmonate; SA;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2014 سفارش ترجمه

The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris

Keywords: Ala; alanine; AP; alkaline phosphatase; BMGY; buffered minimal glycerol-complex medium; BMMY; buffered minimal methanol-complex medium; CalB; Candida antarctica lipase B; DMSO; dimethyl sulfoxide; dNTP; deoxyribonucleoside triphosphate; ER; endoplasmic re

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris - functional and kinetic asymmetry revealed in proteoliposomes

Keywords: MeAIB; a-(methylamino)isobutyric acid; BCH; 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid; C12E8; octaethylene glycol monododecyl ether; YPDS; Yeast Extract Peptone Dextrose Sorbitol; BMGY; Buffered Glycerol-complex Medium; DDM; n-dodecyl-beta-D-maltos

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Isolation and characterization of novel variants of BBI coding genes from the legume Lathyrus sativus

Keywords: Grass pea; Bowman-Birk isoforms; Proteolytic enzymes; Reactive site; Recombinant proteins; Inhibitory properties; Comparative modeling; APNE; N-acetyl-DL-phenylalanine-ß-naphthyl estere; BApNA; N-benzoyl-DL-arginine-p-nitroanilide; BBIs; Bowman-Birk

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2012 سفارش ترجمه

Heterologous production and characterisation of two distinct dihaem-containing membrane integral cytochrome b561 enzymes from Arabidopsis thaliana in Pichia pastoris and Escherichia coli cells

Keywords: Ax; absorption at a wavelength of x nm; BCA; bicinchoninic acid; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; BSA; bovine serum albumin; CV; column volumes; CYBASC; cytochrome b561 ascorbate reducible family; cyt b561; c

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2012 سفارش ترجمه

Cloning, characterization and mutagenesis of Russell's viper venom l-amino acid oxidase: Insights into its catalytic mechanism

Keywords: l-Amino acid oxidase; Geographic variation; Kinetic specificity; Mutagenesis; Russell's viper (Daboia siamensis, Daboia russelii); BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; DAAO; d-amino acid oxidase; FAD; flavin ad

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2012 سفارش ترجمه

The chromophore structure of the long-lived intermediate of the C128T channelrhodopsin-2 variant

Keywords: ChR2; channelrhodopsin-2; BR; bacteriorhodopsin; BMGY; buffered glycerol-complex medium; BMMY; buffered methanol-complex medium; DDM; n-dodecyl β-d-maltoside; RR; resonance Raman; Channelrhodopsin; Photocycle; Retinal; Retroretinal; Resonance Raman; Bact

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2011 سفارش ترجمه

Comparative study of mature and zymogen mite cysteine protease stability and pH unfolding

Keywords: AMC; 7-amino-4-methylcoumarin; ANS; 8-anilino-1-naphthalenesulfonic acid; BMGY; Yeast Glycerol Buffered Media; Boc; N-t-butoxy-carbonyl; C34A; replacement of the catalytic cysteine (C34) by an alanine; DTT; dithiotreitol; E-64; L-trans-epoxysuccinyl-leucy

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2010 سفارش ترجمه

Expression of an anti-CD33 single-chain antibody by Pichia pastoris

Keywords: Abs; antibodies; AML; acute myeloid leukaemia; AOX; alcohol oxidase; BMGY; buffered glycerol complex medium; BMMY; buffered methanol complex medium; CFU-GEMM; colony forming units for granulocytes, erythrocytes, monocytes and megakaryocytes; CFU-GM, BFU-E

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2005 سفارش ترجمه