
Keywords: اتصال Ca2 +; Centrin-1; Intein-tag; Tyrosine fluorescence; Ca2+ binding; Nonspecific fluorescence; HsCentrin-1; Homo sapiens Centrin-1; DTT; Dithiothreitol; HIC; Hydrophobic Interaction Chromatography; IMPACT; Intein-mediated purification with an affinity chitin-bindi