کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2051332 | 1074197 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Importance of a repetitive nine-residue sequence motif for intracellular stability and functional structure of a family I.3 lipase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
PML5 is a functional derivative of a family I.3 lipase from Pseudomonas sp. MIS38 and contains five repeats of a nine-residue sequence motif. Two aspartate residues within the second and third repetitive sequences of PML5 were replaced by Ala. The secretion level, intracellular accumulation level, and stability of the resultant mutant protein were greatly reduced as compared to those of PML5. In addition, this mutant protein was inactive and did not bind Ca2+ ion. We propose that the repetitive sequences of PML5 form a β-roll structure in the cells and thereby contribute to the intracellular stability and secretion efficiency of the protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 21, 29 August 2005, Pages 4707–4712
Journal: FEBS Letters - Volume 579, Issue 21, 29 August 2005, Pages 4707–4712
نویسندگان
Clement Angkawidjaja, Aditya Paul, Yuichi Koga, Kazufumi Takano, Shigenori Kanaya,