
Flow cytometry-based methods for studying signaling in human CD4+CD25+FOXP3+ T regulatory cells
Keywords: ERK; extracellular regulated kinase; FACS; flourescence activated cell sorter; Treg; T regulatory; nTreg; naturally-occurring Treg; PBMCs; peripheral blood mononuclear cells; PI3′K; phosphatidyl inositide-3 kinase; PHA; phytohemagglutinin; MAPK; Ras/ mito