کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9194563 | 1580504 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CDR3 sequence preference of TCRBV8S2+ T cells within the CNS does not reflect single amino acid dependent avidity expansion
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کلمات کلیدی
EAERT1TCRBVFACSTCrMBPCDREAE/MS - EAE / MSexperimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیautoimmunity - خودایمنیGuinea pig - خوک گینه، خوکچه هندیCNS - دستگاه عصبی مرکزیT cells - سلول های تیcentral nervous system - سیستم عصبی مرکزیfluorescence activated cell sorter - فلورسانس سلول فعال شده سلولMHC - مجموعه سازگاری بافتی اصلیcomplementary determining region - منطقه تعریف مکملMyelin basic protein - پروتئین پایه میلینT cell receptor - گیرنده سلول TT cell receptors - گیرنده های T سلول
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: CDR3 sequence preference of TCRBV8S2+ T cells within the CNS does not reflect single amino acid dependent avidity expansion CDR3 sequence preference of TCRBV8S2+ T cells within the CNS does not reflect single amino acid dependent avidity expansion](/preview/png/9194563.png)
چکیده انگلیسی
To investigate the influence of antigen and restricting MHC class II molecule on the T cell repertoire, we varied the peptide source by immunizing either with myelin basic protein (MBP) rat63-88 or MBPGUINEA PIG (GP)63-88, which differ in the core region of the peptide binding site at position 79 by a single exchange of threonine (T) to serine (S) and by altering the MHC by immunizing MHC congenic LEW (RT1l) and LEW.1W (RT1u) rats. In both MHC haplotypes both peptides lead to oligoclonal dominance of TCRBV8S2 expressing T cells within the central nervous system (CNS) as assessed by complementary determining region 3 (CDR3) spectratyping. In contrast cytofluorometric analysis indicated that only MBPGP63-88 in context with the RT1l haplotype of the LEW rat lead to strong expansions of TCRBV8S2 expressing T cells within the CNS. Importantly, the small conservative change from S to T at position 79 within MBP63-88 had a strong influence both on the encephalitogenic potential of the peptide and on the number of TCRBV8S2+ T cells infiltrating the CNS. These results demonstrate that even minor changes in only one side chain of an amino acid within an (auto)antigen can dramatically alter TCR avidity for certain MHC class II/peptide complexes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 166, Issues 1â2, September 2005, Pages 47-54
Journal: Journal of Neuroimmunology - Volume 166, Issues 1â2, September 2005, Pages 47-54
نویسندگان
Katrien L. de Graaf, Gabrielle Paulsson Berne, Martin M. Herrmann, Göran K. Hansson, Tomas Olsson, Robert Weissert,