LC-MS-based Metabolomics in Traditional Chinese Medicines Research: Personal Experiences
Keywords: LC-MS; metabolomics; traditional Chinese medicines;
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Opening the s-triazine ring and biuret hydrolysis during conversion of atrazine by Frankia sp. strain EuI1c
Keywords: Frankia; s-triazine ring-cleavage; Reverse genetics; qRT PCR; atzD; Atrazine D; trzD; Triazine D; atzE; Atrazine E; trzE; Triazine E; LC-MS; Liquid Chromatography-Mass Spectrometry; AtzDEF; Atrazine gene D, E and F; TrzDEF; Tirazine gene D, E and F; atzR;
Production of theobromine by N-demethylation of caffeine using metabolically engineered E. coli
Keywords: LB; Luria-Bertani broth; TB; Terrific Broth; SB; Super broth; PX; Paraxanthine; HPLC; High pressure liquid chromatography; LC-MS; Liquid Chromatography-Mass spectrophotometry; NMR; Nuclear Magnetic Resonance; SDS-PAGE; Sodium dodecyl sulfate - Polyacrylam
Differential phenolic production in leaves of Vitis vinifera cv. Alvarinho affected with esca disease
Keywords: Esca; Flavonoids; Grapevine; High performance liquid chromatography (HPLC); Hydroxycinnamoyl tartaric acids; Principal components analysis (PCA); Vitis vinifera; ahl; apparently healthy leaves; dl; diseased leaves; hl; healthy leaves; HPLC-DAD-MS; high pe
Metabolomic analysis of urine samples by UHPLC-QTOF-MS: Impact of normalization strategies
Keywords: Metabolomics; LC-MS; Urine; Normalization; Kidney failure;
In Vitro and In Situ Characterization of Triterpene Glycosides From Cimicifuga racemosa Extract
Keywords: Biopharmaceutics Classification System (BCS); cell culture; dissolution; solubility; gastrointestinal; LC-MS; absorption; Caco-2 cells; natural products; Ze 450; Cimicifuga racemosa extract; BCS; Biopharmaceutics Classification System; ab; apical-to-baso
Feasibility of the functional expression of the human organic anion transporting polypeptide 1B1 (OATP1B1) and its genetic variant 521T/C in the mouse liver
Keywords: ALP; alkaline phosphatase; ALT; alanine aminotransferase; AST; aspartate aminotransferase; AUC; the area under the plasma concentration-time curve; CLapp, hep; hepatic uptake clearance; hOATP1B1; human organic anion transporting polypeptide 1B1; HGD; hydr
THMGUI: A user-friendly Matlab graphical user interface of Tchebichef-Hermite moment method for handing three-dimensional landscapes
Keywords: Tchebichef-Hermite moment method; Graphical user interface; THMGUI; LC-MS; 3D landscapes; Quantitative analysis;
The assessment of the chronic hepatotoxicity induced by Polygoni Multiflori Radix in rats: A pilot study by using untargeted metabolomics method
Keywords: TCM; Traditional Chinese Medicine; HSW; He-Shou-Wu; DILI; Drug-Induced Live Injury; NAFLD; Non-Alcoholic Fatty Liver Disease; LC-MS; Liquid-Chromatography-Tandem Mass Spectrometry; GC-MS; Gas Chromatography-Mass Spectrometry; TSG; 2,3,5,4â²-tetrahydr
Dolichospermum and Aphanizomenon as neurotoxins producers in some Russian freshwaters
Keywords: Cyanotoxins; LC-MS; PCR; Neurotoxin producers; Aphanizomenon; Dolichospermum;
The binding effectiveness of anti-r-disintegrin polyclonal antibodies against disintegrins and PII and PIII metalloproteases: An immunological survey of type A, B and AÂ +Â B venoms from Mohave rattlesnakes
Keywords: SVMP; snake venom metalloprotease; ELISA; Enzyme-Linked Immunosorbent Assay; ARDPA; anti-recombinant disintegrin polyclonal antibody; r-Mojastin 1; recombinant mojastin 1; ADAM; a disintegrin and metalloprotease; LD50; Lethal Dose 50; LC-MS; Liquid Chroma
Ergothioneine stands out from hercynine in the reaction with singlet oxygen: Resistance to glutathione and TRIS in the generation of specific products indicates high reactivity
Keywords: 1O2; singlet oxygen; DHPN; N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalenedipropanamide; DHPNO2; DHPN 1,4-endoperoxide; ET; ergothioneine; ETT; ergothioneine transporter; GSH; glutathione; HPLC; high-performance liquid chromatography; HRMS; accurate ion mass
Convergence of eicosanoid and integrin biology: Role of Src in 12-LOX activation
Keywords: HETE; hydroxyeicosatetraenoic acid; LN; laminin; RTK; receptor tyrosine kinase; AA; arachidonic acid; pSFK; phospho Src family kinase; AA; Arachidonic acid; PTPase; protein tyrosine phosphatase; IP; immunoprecipitation; LC-MS; Liquid chromatography-mass s
Proteomics offers insight to the mechanism behind Pisum sativum L. response to pea seed-borne mosaic virus (PSbMV)
Keywords: Proteome; Pea seed-borne mosaic virus PSbMV; Potyvirus; Resistance; LC-MS;
Dammarane-type saponins from leaves of Ziziphus spina-christi
Keywords: Ziziphus spina-christi; Rhamnaceae; Dammarane saponins; Lotogenin; LC-MS;
Age dependent neuroprotective effects of medhya rasayana prepared from Clitoria ternatea Linn. in stress induced rat brain
Keywords: BER; Base Excision Repair; C; Control animals; CA3; Cornus Ammonis; CSI; Control animals subjected to Stereotaxic Injury; DNA; Deoxyribonucleic Acid; GR; Glutathione Reductase; HPLC; High Performance Liquid Chromatography; LC3; Light Chain 3; LC-MS; Liqui
Purification and characterization of angiotensin-converting enzyme-inhibitory peptides from Nile tilapia (Oreochromis niloticus) skin gelatine produced by an enzymatic membrane reactor
Keywords: Enzyme membrane reactor; Fish skin; Collagen; Hydrolysis; Peptides; LC-MS;
Peptide identification in a porcine gelatin prolyl endoproteinase hydrolysate with angiotensin converting enzyme (ACE) inhibitory and hypotensive activity
Keywords: Bioactive peptides; LC-MS; Gelatin hydrolysate; Angiotensin converting enzyme; Aspergillus niger prolyl endoproteinase (An-PEP); Protein hydrolysate; Spontaneously hypertensive rats;
Proteomic profiling reveals DNA damage, nucleolar and ribosomal stress are the main responses to oxaliplatin treatment in cancer cells
Keywords: Oxaliplatin; Proteomics; LC-MS; Nucleolar stress; Ribosomal stress;
Levothyroxine sodium revisited: A wholistic structural elucidation approach of new impurities via HPLC-HRMS/MS, on-line H/D exchange, NMR spectroscopy and chemical synthesis
Keywords: Levothyroxine; Thyroid hormone; Pharmaceutical impurities; On-line H/D Exchange; HDX; LC-MS;
Terminalia arjuna bark extract attenuates picrotoxin-induced behavioral changes by activation of serotonergic, dopaminergic, GABAergic and antioxidant systems
Keywords: Anxiety; Terminalia arjuna; Gene expression; Picrotoxin; Neurotransmitters; LC-MS;
Recent progress, challenges and trends in trace determination of drug analysis using molecularly imprinted solid-phase microextraction technology
Keywords: AA; Ascorbic Acid; AAS; Anabolic steroids; ABA; Abacavir; BPA; Bisphenol A; CCD; Central composite design; CE; Capillary electrophoresis; CGA; Chlorogenic acid; CGC; Capillary gas chromatography; CMIP; Conducting molecularly-imprinted polymer; CNT; Carbon
(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways
Keywords: PDM; (Poly)phenol Digested Metabolites; ROS; reactive oxygen species; GAE; gallic acid equivalents; ORAC; Oxygen Radical Absorbance Capacity; LC-MS; Liquid Chromatography Mass Spectrometry; CSM; complete supplement mixture; R.F.U.; relative fluorescence u
The silymarin composition⦠and why does it matter???
Keywords: ADME; absorption, distribution, metabolism, and excretion; ASTM; American Society for Testing and Materials; NHDF; normal human dermal fibroblasts; NHEK; normal human epidermal keratinocytes; PE; petroleum ether; LC-MS; liquid chromatography-mass spectr
Fast pure ion chromatograms extraction method for LC-MS
Keywords: Pure ion chromatogram; LC-MS; Feature extraction;
Roles of Sphincter of Oddi Laxity in Bile Duct Microenvironment in Patients with Cholangiolithiasis: From the Perspective of the Microbiome and Metabolome
Keywords: LC-MS; liquid chromatography mass spectrometry; OTU; operational taxonomic unit; rRNA; ribosomal RNA; SOL; sphincter of Oddi laxity; TIC; total ion chromatogram; VIP; variable importance in projection;
Identification of related substances in tofacitinib citrate by LC-MS techniques for synthetic process optimization
Keywords: Tofacitinib citrate; Process-related substances; Degradation products; Synthetic process optimization; LC-MS;
Identification of post-translational modifications of Aβ peptide in platelet membranes from patients with cerebral amyloid angiopathy
Keywords: CAA; cerebral amyloid angiopathy; AD; Alzheimer's disease; ThT; thioflavin T; PTMs; post-translational modifications; Aβ; amyloid-β; UPLC/ESI-Q-TOF/MS; ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectro
An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR
Keywords: B&W; washing and binding; CAP; chloramphenicol; Ct; cycle threshold; dsDNA; double stranded DNA; ELISA; enzyme linked immunosorbent assay; EU; European Union; FF; florfenicol; FRET; fluorescence resonance energy transfer; GC; gas chromatography; LC; liqui
Multiway analysis methods applied to the fluorescence excitation-emission dataset for the simultaneous quantification of valsartan and amlodipine in tablets
Keywords: 3W-PLS; three-way partial least squares; ALS; alternating least squares; AML; amlodipine besylate; ANOVA; one-way analysis of variance; DAD; diode array detector; EEM; excitation-emission matrix; GRAM; generalized rank annihilation method; HCl; hydrochlor
In vitro CYP-mediated drug metabolism in the zebrafish (embryo) using human reference compounds
Keywords: Zebrafish; Cytochrome; Microsomes; Ontogeny; Sex; LC-MS;
Fast characterization of C-glycoside acetophenones in Medemia argun male racemes (an Ancient Egyptian palm) using LC-MS analyses and computational study with their antioxidant effect
Keywords: Medemia argun; Male racemes; C-glycosides acetophenones; LC-MS; DFT; Fukui function;
R- and S-Warfarin Were Transported by Breast Cancer Resistance Protein: From In Vitro to Pharmacokinetic-Pharmacodynamic Studies
Keywords: ABC transporters; drug transport; mass spectrometry; preclinical pharmacokinetics; pharmacokinetics/pharmacodynamics; INR; international normalized ratio; BCRP; breast cancer resistance protein; ABCG2; ATP-binding cassette transporter G2; CYP450; cytochro
E-configuration structures of EPA and DHA derived from Euphausia superba and their significant inhibitive effects on growth of human cancer cell lines in vitro
Keywords: DHA; docosahexaenoic acid; EPA; eicosapentaenoic acid; HRESIMS; high resolution electrospray ionization mass spectroscopy; NMR; nuclear magnetic resonance; HPLC; high performance liquid chromatography; TLC; thin layer chromatography; GC-MS; gas chromatogr
Galloylquinic acid derivatives from Copaifera langsdorffii leaves display gastroprotective activity
Keywords: Galloylquinic acid; Phenol; Copaiba; Gastroprotection; Adenocarcinoma; Ulcer; HPLC; high-performance liquid chromatography; LC-MS; liquid chromatography coupled to mass spectrometry; NMR; nuclear magnetic resonance; HSQC; heteronuclear single quantum cohe
Thiol dioxygenase turnover yields benzothiazole products from 2-mercaptoaniline and O2-dependent oxidation of primary alcohols
Keywords: Non-heme mononuclear iron enzymes; Thiol dioxygenase; Alcohol oxidation; Benzothiazoles; CDO; cysteine dioxygenase; MDO; 3-mercaptopropionic acid dioxygenase; ROS; reactive oxygen species; Mm; Mus musculus; Av; Azotobacter vinelandii; 2ma; 2-mercaptoanili
Applying 'omics technologies in chemicals risk assessment: Report of an ECETOC workshop
Keywords: Regulatory toxicology; Transcriptomics; Metabolomics; Good laboratory practice (GLP); Weight-of-evidence (WoE); Adverse outcome pathway (AOP); Mode-of-action (MoA); Gene expression; Differentially expressed genes; ANOVA; Analysis of variance; AOP; Adverse
Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds
Keywords: Acr-dG; 1,N2-propanodeoxyguanosine; AGEs; advanced glycation end-product; ALEs; advanced lipoxidation end product; ApoB100; apolipoprotein B100; ApoE; apolipoprotein E; ARP; aldehyde reactive probe; AspN; endoproteinase AspN (flavastacin); βLG; β-lactog
FASN Inhibition and Taxane Treatment Combine to Enhance Anti-tumor Efficacy in Diverse Xenograft Tumor Models through Disruption of Tubulin Palmitoylation and Microtubule Organization and FASN Inhibition-Mediated Effects on Oncogenic Signaling and Gene Ex
Keywords: NSCLC; Non-small-cell lung cancer; TGI; Tumor growth inhibition; MEM; Minimal essential media; DMEM; Dulbecco's modified Eagle medium; FBS; Fetal bovine serum; CS-FBS; Charcoal-filtered FBS-fetal bovine serum; IMDM; Iscove's modified Eagle medium; LC-MS
Isoprostanes, neuroprostanes and phytoprostanes: An overview of 25Â years of research in chemistry and biology
Keywords: Isoprostanes; Neuroprostanes; Phytoprostanes; Lipid Peroxidation; Polyunsaturated fatty acids; AA; Arachidonic acid; AD; Alzheimer's disease; AdA; Adrenic acid; ALA; α-Linolenic acid; APCI; Atmospheric pressure chemical ionization; BHT; Butylated hydroxy
Antibacterial screening of Juncaceae species native to the Carpathian Basin against resistant strains and LC-MS investigation of phenanthrenes responsible for the effect
Keywords: Juncaceae; Juncus; Luzula; Anti-MRSA activity; Phenanthrenes; LC-MS;
Quantitative determination of metaxalone in human plasma by LC-MS and its application in a pharmacokinetic study
Keywords: Metaxalone; LC-MS; Pharmacokinetics
Pharmacokinetic profiles of falcarindiol and oplopandiol in rats after oral administration of polyynes extract of Oplopanax elatus
Keywords: Oplopanax elatus; Polyynes; LC-MS; Pharmacokinetic study;
A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I
Keywords: Sialyltransferase; Michaelis-Menten; Product inhibition; Mass spectrometry; Gal; Galactose; GalNAc; N-acetylgalactosamine; GlcNAc; N-acetylglucosamine; CMP-[14C]Neu5Ac; C14-labeled cytidine 5â²-monophosphate N-acetylneuraminic acid; CMP-Neu5Gc; Cytidin
Impact of microbial derived secondary bile acids on colonization resistance against Clostridium difficile in the gastrointestinal tract
Keywords: Clostridium difficile; Secondary bile acids; Colonization resistance; Gut microbiota; Antibiotics; GI; Gastrointestinal; CDI; Clostridium difficile infection; LC-MS; Liquid chromatography mass spectrometry; CA; cholate; CDCA; chenodeoxycholate; DCA; deoxy
A simple LC-MS method for the determination of iohexol and iothalamate in serum, using ioversol as an internal standard
Keywords: LC-MS; Iohexol; Iothalamate; Ioversol; Glomerular filtration rate; Nephrology;
Identification and characterization of human UDP-glucuronosyltransferases responsible for the in vitro glucuronidation of ursolic acid
Keywords: Ursolic acid; UDP-glucuronosyltransferases; Metabolism pathway; Metabolism characterization; UA; ursolic acid; HLMs; human liver microsomes; HIMs; human intestinal microsomes; UGTs; UDP-glucuronosyltransferases; DDI; drug-drug interactions; RILD; Resear
Recombinant expression, refolding, purification and characterization of Pseudomonas aeruginosa protease IV in Escherichia coli
Keywords: Pseudomonas aeruginosa; Protease IV; Recombinant expression; Refolding; Purification; Activity; TRX; thioredoxin; LB medium; Luria-Bertani medium; ORF; open reading frame; DO; dissolved oxygen; FCM; fermentation complex medium; IPTG; isopropyl thio-β-d-g
Enhancement of downy mildew disease resistance in pearl millet by the G_app7 bioactive compound produced by Ganoderma applanatum
Keywords: Anti-oomycete compound; Downy mildew disease resistance; Ganoderma applanatum; LC-MS; Pearl millet; Sclerospora graminicola; 2D NMR
A feasibility study of UHPLC-HRMS accurate-mass screening methods for multiclass testing of organic contaminants in food
Keywords: Pesticide; Food; LC-MS; Screening methods