An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR

A novel sensitive approach for detection of CAP was designed based on CAP specific aptamer and qRT-PCR. The established aptamer-based detection system exhibited a high sensitivity toward CAP with a limit of detection of 0.1 ng/mL. This aptamer-based detection system also showed high selectivity against interference molecules TAP and FF, which are CAP's structure analogs. Moreover, this detection system was further used to detect CAP in real spiked milk, and the recovery rate of CAP from spiked milk samples ranged from 94.0% to 102.0%.98