کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8273071 | 1535078 | 2017 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of post-translational modifications of Aβ peptide in platelet membranes from patients with cerebral amyloid angiopathy
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کلمات کلیدی
PBSMPVPDWPLTPTMsAPPLC-MSAβCAAP-LCRMSRamyloid-β - آمیلوئید βcerebral amyloid angiopathy - آنژیوپاتی آمیلوئید مغزیOlder people - افراد سالخورده یا مسنMethionine oxidation - اکسیداسیون متیونینThT - بلهAlzheimer's disease - بیماری آلزایمرpost-translational modifications - تغییرات پس از ترجمه، پیرایش پساترجمهThioflavin T - تیوفلاوین TPlatelet count - شمارش پلاکتPlatelet distribution width - عرض توست پلاکتphosphate buffer saline - فسفات بافر شورmean platelet volume - متوسط حجم پلاکتmethionine sulfoxide reductase - متیونین سولفوکسید ردوکتازMolecular weight - وزن مولکولیamyloid precursor protein - پروتئین پیش ماده آمیلوئیAβ peptide - پپتید Aβ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cerebral amyloid angiopathy (CAA) is characterized by cerebrovascular amyloid deposition. It contributes to the rate of cognitive decline in older individuals and is present in > 90% of patients with Alzheimer's disease (AD), with no cure so far. Molecular modifications during CAA should be elucidated to improve its diagnosis and treatment. In this study, amyloid-β (Aβ) aggregates in platelet membranes from 65 patients with CAA and 66 healthy volunteers (controls) were confirmed through thioflavin T (ThT) assay and Western blot analysis. Further, post-translational modifications (PTMs) of Aβ in platelet membranes were analyzed using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS). ThT assay results indicated that there were amyloid components in platelets from both patients with CAA and controls. Western blot analysis showed that different molecular weight (MW) Aβ aggregates were found in platelet membranes. LC-MS analysis showed that PTMs including methylation, phosphopantetheine, phosphorylation, deamidation, and acetylation, occurred in Aβ peptide in platelet membranes from both patients with CAA and controls, while Met35 oxidation (MetOX) and Gln15 deamidation were identified only from patients with CAA. Thus, this study identified potential biomarkers of CAA and characterized the mechanism underlying amyloidogenesis in CAA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Neurological Sciences - Volume 383, 15 December 2017, Pages 11-17
Journal: Journal of the Neurological Sciences - Volume 383, 15 December 2017, Pages 11-17
نویسندگان
Lin Song, Rong Xue, Peng Ge, Man Li, Liang Wang, Fang Zheng, Lili Zhao, Zhexiang Wang, Zhuowei Wang, Qianqian Wang, Ning Liu, Xuguo Sun,