Studying G protein-coupled receptor activation using split-tobacco etch virus assays
Keywords: لوسیفراز; Split-TEV; Cell-based GPCR assay; β-Arrestin; Luciferase; Primary cells
مقالات ISI لوسیفراز (ترجمه نشده)
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Stepwise synthesis of oligonucleotide–peptide conjugates containing guanidinium and lipophilic groups in their 3′-termini
Keywords: لوسیفراز; ODN–peptide conjugates; Dickerson–Drew dodecamer; Luciferase; Postsynthetic guanidinylation reaction; Ureas; Antisense oligonucleotide; Phosphorothioates
Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
Keywords: لوسیفراز; Human circadian rhythm; Human biological clock; Mononuclear cells; Clock genes; Self-sustained circadian rhythm; Luciferase;
Noninvasive bioluminescence imaging of c-fos expression in the mouse barrel cortex
Keywords: لوسیفراز; c-fos; Bioluminescence; Barrel cortex; Luciferase; Mouse; Immediate early genes
Evaluation of HIV-1 integrase inhibitors on human primary macrophages using a luciferase-based single-cycle phenotypic assay
Keywords: لوسیفراز; Integrase; Integrase inhibitors; Macrophages; Lentiviral vectors; Luciferase;
Enhanced electro-mediated gene delivery using carrier genes
Keywords: لوسیفراز; Transfection; Electroporation; Luciferase; Gene delivery; Gene carrier
Trigger factor lacking the PPIase domain can enhance the folding of eukaryotic multi-domain proteins in Escherichia coli
Keywords: لوسیفراز; Trigger factor; Ribosome; Chaperone; Luciferase; Multi-domain
Phytoestrogens from Psoralea corylifolia reveal estrogen receptor-subtype selectivity
Keywords: لوسیفراز; Psoralea corylifolia; Estrogen receptor (ER); Luciferase; Phytoestrogen
von Hippel–Lindau β-domain–luciferase fusion protein as a bioluminescent hydroxyproline sensor for a hypoxia-inducible factor prolyl hydroxylase assay
Keywords: لوسیفراز; HIF prolyl hydroxylase; HIF prolyl hydroxylase activity assay; Luciferase; von Hippel–Lindau; Recombinant VHL–luciferase fusion protein; Hypoxia-inducible factor
Improvement of the monitoring and biosafety of encapsulated cells using the SFGNESTGL triple reporter system
Keywords: لوسیفراز; Alginate; Microcapsule; TGL; Luciferase; Monitoring
Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring
Keywords: لوسیفراز; Protein transduction domain; Luciferase; Quantification; Cellular uptake; In vivo tracking
RACE-based amplification of cDNA and expression of a luciferin-regenerating enzyme (LRE): An attempt towards persistent bioluminescent signal
Keywords: لوسیفراز; CBHT, 2-cyano-6-hydroxybenzothiazole; NLH, NCL-1/HT2A/LIN-41 repeat; SGL, SMP-30/gluconolaconase/LRE-like region; PTS, peroxisomal targeting sequence; SMP-30, senescence marker protein 30Luciferin-regenerating enzyme (LRE); Oxyluciferin; Luciferase; Firef
Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria
Keywords: لوسیفراز; Antimicrobial peptide; Expression; Purification; Recombinant; Fusion protein; Permeabilization; Luciferase; Sea urchin
Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I – Thymidine kinase/ganciclovir in glioma
Keywords: لوسیفراز; Glioma; HSV1-tk; Luciferase; Molecular imaging; Suicidal gene therapy
Red-emitting luciferases for bioluminescence reporter and imaging applications
Keywords: لوسیفراز; Bioluminescence; Codon-optimized; Color; Firefly; Imaging; Luciferase; Mutagenesis; Red; Reporter;
The design and validation of a novel phenotypic assay to determine HIV-1 coreceptor usage of clinical isolates
Keywords: لوسیفراز; Chemokine receptor; Luciferase; Pseudovirus; Viral fitness; HIV; Viral tropism; Coreceptor usage; CCR5; CXCR4;
Research ArticlePorphobilinogen deaminase over-expression in hepatocytes, but not in erythrocytes, prevents accumulation of toxic porphyrin precursors in a mouse model of acute intermittent porphyria
Keywords: لوسیفراز; Acute intermittent porphyria; Acute attack; Porphyrin precursor excretion; Motor coordination; Bone marrow transplant; Liver gene transfer; AIP; acute intermittent porphyria; PBGD; porphobilinogen deaminase; ALA; δ-aminolevulinic acid; LUC; luciferase; P
The detection of dioxin- and estrogen-like pollutants in marine and freshwater fishes cultivated in Pearl River Delta, China
Keywords: لوسیفراز; Luciferase; MCF7; Real-time PCR; Western blot; Colony formation assay
Foxj3 transcriptionally activates Mef2c and regulates adult skeletal muscle fiber type identity
Keywords: لوسیفراز; Fox; Forkhead box; FBS; forkhead binding site; Mef2c; Myocyte enhancer factor 2c; luc; luciferase; β-gal; β-galactosidase; bHLH; basic Helix-Loop-Helix; H&E; hemotoxylin and eosin; NFR; nuclear fast red; WT; wild type; GFP; green fluorescent protein; DM
Coordinated up-regulation of cyclooxygenase-2 and microsomal prostaglandin E synthase 1 transcription by nuclear factor kappa B and early growth response-1 in macrophages
Keywords: لوسیفراز; AA; arachidonic acid; ChIP; chromatin immunoprecipitation; COX; cyclooxygenase; Egr-1; early growth response 1; LUC; luciferase; PG; prostaglandin; mPGES-1; microsomal Prostaglandin E synthase 1; cPGES; cytosolic Prostaglandin E synthase; PGHS; Prostaglan
Circadian oscillators in the epithalamus
Keywords: لوسیفراز; lateral habenula; medial habenula; ependymal, period 2; electrophysiology; bioluminescence; cpm; counts per minute; Hb; habenula; LD; light/dark cycle; LHb; lateral habenula; LHbL; lateral portion of the lateral habenula; LHbM; medial portion of the later
Cox-2 gene expression in chemically induced skin papillomas cannot predict subsequent tumor fate
Keywords: لوسیفراز; Cyclooxygenase; Luciferase; Knock-in mouse; Gene expression; Skin cancer; DMBA/TPA;
Ligand-modified gene carriers increased uptake in target cells but reduced DNA release and transfection efficiency
Keywords: لوسیفراز; Controlled release; Gene delivery; Luciferase; Megalin; Polymer particle
A cell-based reporter assay for inhibitor screening of hepatitis C virus RNA-dependent RNA polymerase
Keywords: لوسیفراز; Hepatitis C virus; RNA-dependent RNA polymerase; Luciferase; High-throughput screening;
Development of quantitative and high-throughput assays of polyomavirus and papillomavirus DNA replication
Keywords: لوسیفراز; Polyomavirus; Papillomavirus; DNA replication; Luciferase; SV40; HPV31; High-throughput; Cell-based assay; Small molecule inhibitors
Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC
Keywords: لوسیفراز; HIV-1; Neutralizing antibody; PBMC assay; HIV reporter virus; Vaccine assessment; Assay standardization; HIV neutralization; Luciferase; Envelope glycoprotein
The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona
Keywords: لوسیفراز; EPM; equine protozoal myeloencephalitis; RACE; rapid amplification of cDNA ends; Fluc; firefly luciferase; Rluc; renilla luciferase; Sarcocystis neurona; Transfection; Promoter; Gene regulation; Apicomplexa; Coccidia; Transcription; Luciferase;
Translationally controlled tumor protein is a novel heat shock protein with chaperone-like activity
Keywords: لوسیفراز; TCTP, translationally controlled tumor protein; CS, citrulline synthase; HSPs, heat shock proteins; PBMC, peripheral blood mononuclear cellsTCTP; Chaperone; Luciferase; Heat shock protein
Analysis of in vitro SUMOylation using bioluminescence resonance energy transfer (BRET)
Keywords: لوسیفراز; SUMO; Energy transfer; BRET; FRET; RanGAP; Luciferase
Improved lux reporters for use in Staphylococcus aureus
Keywords: لوسیفراز; Gram-positive bacteria; Fatty acid aldehyde; FMN reductase; Luciferase; Shuttle vector; Transcription reporter
Reconstitution of blue fluorescent protein from recombinant apoaequorin and synthetic coelenteramide
Keywords: لوسیفراز; Apoaequorin; apoprotein of aequorin; BFP; blue fluorescent protein of aequorin; gFP; greenish fluorescent protein; syn-BFP; blue fluorescent protein reconstituted from apoaequorin and coelenteramide in the presence of calcium; syn-gFP; greenish fluorescen
Transcription of the mitochondrial citrate carrier gene: Identification of a silencer and its binding protein ZNF224
Keywords: لوسیفراز; CIC; citrate carrier; Kap1; KRAB-associated protein 1; KRAB; Krüppel-associated box; LUC; luciferase; ZNF224; zinc finger protein 224; Citrate carrier; Gene expression; Mitochondria; Promoter; Silencer; Transcription; ZNF224;
Activation of the GDNF-inducible transcription factor (GIF) gene promoter by glucocorticoid and progesterone
Keywords: لوسیفراز; CAT, chloramphenicol acetyltransferase; ERE, estrogen-responsive element; HEO, human estrogen receptor; HGO, human glucocorticoid receptor; hPro, human progesterone receptorGlucocorticoid; Progesterone; GIF promoter; Luciferase; GRE/PRE
Development of a cell-based assay for high-throughput screening of inhibitors against HCV genotypes 1a and 1b in a single well
Keywords: لوسیفراز; HTS; HCV; Replicon; Cell-based assay; SEAP; Luciferase; Resazurin cytotoxicity assay
Establishment of a chimeric reporting system for the universal detection and high-throughput screening of G protein-coupled receptors
Keywords: لوسیفراز; G protein-coupled receptors; Luciferase; SRE; CRE; Gqi
Pulmonary gene delivery of hybrid vector, lipopolyplex containing N-lauroylsarcosine, via the systemic route
Keywords: لوسیفراز; Hybrid vector; Pulmonary gene delivery; Luciferase; Polyethylenimine; N-lauroylsarcosine
The effect of high intensity focused ultrasound on luciferase activity on two tumor cell lines in vitro, under the control of a CMV promoter
Keywords: لوسیفراز; Tumor cell lines; Luciferase; CMV promoter; Thermal stress; HIFU; Viability;
The evolution of the adenylate-forming protein family in beetles: Multiple luciferase gene paralogues in fireflies and glow-worms
Keywords: لوسیفراز; Luciferase; Lampyridae; Tribolium castaneum; Adenylate-forming enzyme superfamily; Purifying selection; Subfunctionalization; Bioluminescence
Polymeric nanoparticles for siRNA delivery and gene silencing
Keywords: لوسیفراز; RNA interference; siRNA; poly(d,l-lactide-co-glycolide); Luciferase; Sustained release
Psammaplin A as a general activator of cell-based signaling assays via HDAC inhibition and studies on some bromotyrosine derivatives
Keywords: لوسیفراز; Wnt signaling; Luciferase; HDAC; Natural product library; LCMS; Bromotyrosine; Psammaplin; N-Methyl glutathione; Disulfide exchange; Reductive methylation; Sulfinate ester
DNA polymerase β and PARP activities in base excision repair in living cells
Keywords: لوسیفراز; Base excision repair; DNA polymerase β; Poly(ADP-ribose) polymerase; Luciferase; Plasmid-based assay;
Mitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells
Keywords: لوسیفراز; Mitochondria; Calcium; Heart; Cardiomyocyte; Pancreas; Liver; ATP; Luciferase; Aequorin
The effect of self-assembling peptide nanofiber scaffolds on mouse embryonic fibroblast implantation and proliferation
Keywords: لوسیفراز; Cell proliferation; In vivo imaging; Luciferase; Nanofiber scaffold
Heme reversibly damps PERIOD2 rhythms in mouse suprachiasmatic nucleus explants
Keywords: لوسیفراز; circadian; tissue culture; desynchronization; luciferase; NR1D1; clock genes; CO; carbon monoxide; HO; heme oxygenase; LPS; lipopolysaccharide; NMP; N-methylprotoporphyrin IX; PPIX; protoporphyrin IX; RI; rhythmicity index; ROSs; reactive oxygen species;
Characterisation of the differentially regulated trout protein 1 (DRTP1) gene in rainbow trout (Oncorhynchus mykiss)
Keywords: لوسیفراز; Quantitative real-time PCR (qPCR); Promoter; Genome walking; Transfection; Luciferase; Ly-6/uPAR
Sp2 regulates interferon-γ-mediated socs1 gene expression
Keywords: لوسیفراز; GAS, γ-activated sequence; IFN, interferon; IFNGR, IFNγ receptor; IL-4 RE, IL-4 responsive element; IRF, IFN-regulated factor; IRF-E, IRF-binding element; SBE, STAT6-binding elementPromoter; siRNA; Transcription factor Sp2; Luciferase; EMSA
Transcriptional regulation of mitochondrial glycerophosphate acyltransferase is mediated by distal promoter via ChREBP and SREBP-1
Keywords: لوسیفراز; Glycerophosphate acyltransferase; Mitochondria; Promoter; Luciferase; EMSA; ChIP; ChREBP; SREBP; Transcriptional regulation
Detection of anabolic androgenic steroid abuse in doping control using mammalian reporter gene bioassays
Keywords: لوسیفراز; Mammalian reporter gene assay; Luciferase; Sport doping; Androgen; Urine; Mixture
“Functional mapping of the promoter region of the GNB2L1 human gene coding for RACK1 scaffold protein”
Keywords: لوسیفراز; DHEA, dehydroepiandrasterone; PMA, phorbol myristate acetate; LPS, lipopolysaccharide; FLOE, fluorescently labeled oligonucleotide extension, cortisol; GRE, glucocorticoids response elementRACK1; DHEA; LPS; PMA; FLOE; Luciferase
Autonomous Hedgehog signalling is undetectable in PC-3 prostate cancer cells
Keywords: لوسیفراز; Hedgehog; PC-3 cells; Autocrine; Prostate cancer; Gli; Luciferase; Cyclopamine