کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1932416 | 1050580 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 394, Issue 2, 2 April 2010, Pages 348–353
Journal: Biochemical and Biophysical Research Communications - Volume 394, Issue 2, 2 April 2010, Pages 348–353
نویسندگان
Mi-Sook Lee, Eun-Hye Kwon, Hoon-Sung Choi, Seung-Hae Kwon, Chul-Hyun Lee, In-sop Shim, Sang-Kyou Lee, Song Her,