Streamlined purification of fluorescently labeled Escherichia coli phosphate-binding protein (PhoS) suitable for rapid-kinetics applications
Keywords: Phosphate-binding protein (PhoS); Fluorescence; Phosphate release; Phosphate Sensor; Rapid-kinetics; LepA/EF4; BME; β-mercaptoethanol; EDTA; ethylenediaminetetraacetic acid; GTP; Guanosine triphosphate; GTPase; Guanosine triphosphatase; HC PhoS; His-tagg