کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815867 | 1058520 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PERK-eIF2α-ATF4 pathway mediated by endoplasmic reticulum stress response is involved in osteodifferentiation of human periodontal ligament cells under cyclic mechanical force
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CREBOsteodifferentiationORFsOCNCMFα-MEMBMP2ATF6TBSTeIF2αinositol requiring enzyme 1IRE1BSPECLeIF2FBSATF4PVDFGFPPMSFERSMOI - MEOsteocalcin - استئوکلسین Endoplasmic reticulum stress - استرس شبکه آندوپلاسمیcAMP response element binding - الگوریتم پاسخ cAMPenhanced chemiluminescence - بهبود شیمیایی لومنTris-buffered saline Tween-20 - تریس بافر Tween-20 سالینAlpha minimum essential medium - حداقل حداقل آلفا متوسطDiabetes mellitus - دیابت قندیfetal bovine serum - سرم جنین گاوPeriodontal ligament cells - سلول های رباط پریودنتالhuman periodontal ligament cells - سلول های لنفاوی پریودنتال انسانbone sialoprotein - سیلوپروتئین استخوانendoplasmic reticulum - شبکه آندوپلاسمی eukaryotic initiation factor 2 - عامل آغاز کننده یوکاریوتی 2Open reading frames - فریم های خواندن را باز کنیدactivating transcription factor 4 - فعال کردن عامل رونویسی 4activating transcription factor 6 - فعال کردن عامل رونویسی 6Phenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMechanical force - نیروی مکانیکیgreen fluorescent protein - پروتئین فلورسنت سبزbone morphogenic protein 2 - پروتئین مورفولوژیک استخوان 2PERK - پرکpolyvinyldifluoride - پلی وینیلدفلوئوریدmultiplicity of infection - چندین عفونت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To prevent excess accumulation of unfolded proteins in endoplasmic reticulum (ER), eukaryotic cells have signaling pathways from the ER to the cytosol or nucleus. These processes are known as the endoplasmic reticulum stress (ERS) response. Protein kinase R like endoplasmic reticulum kinase (PERK) is a major transducer of the ERS response and it directly phosphorylate α-subunit of eukaryotic initiation factor 2 (eIF2α), resulting in translational attenuation. Phosphorylated eIF2α specifically promoted the translation of the activating transcription factor 4 (ATF4). ATF4 is a known important transcription factor which plays a pivotal role in osteoblast differentiation and bone formation. Furthermore, ATF4 is a downstream target of PERK. Studies have shown that PERK-eIF2α-ATF4 signal pathway mediated by ERS was involved in osteoblastic differentiation of osteoblasts. We have known that orthodontic tooth movement is a process of periodontal ligament cells (PDLCs) osteodifferentiation and alveolar bone remodeling under mechanical force. However, the involvement of PERK-eIF2α-ATF4 signal pathway mediated by ERS in osteogenic differentiation of PDLCs under mechanical force has not been unclear. In our study, we applied the cyclic mechanical force at 10% elongation with 0.5 Hz to mimic occlusal force, and explored whether PERK-eIF2α-ATF4 signaling pathway mediated by ERS involved in osteogenic differentiation of PDLCs under mechanical force. Firstly, cyclic mechanical force will induce ERS and intensify several osteoblast marker genes (ATF4, OCN, and BSP). Next, we found that PERK overexpression increased eIF2α phosphorylation and expression of ATF4, furthermore induced BSP, OCN expression, thus it will promote osteodifferentiation of hPDLCs; mechanical force could promote this effect. However, PERKâ/â cells showed the opposite changes, which will inhibit osteodifferentiation of hPDLCs. Taken together, our study proved that PERK-eIF2α-ATF4 signaling pathway mediated by ERS involved in osteoblast differentiation of PDLCs under cyclic mechanical force.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 8, August 2016, Pages 880-886
Journal: Cellular Signalling - Volume 28, Issue 8, August 2016, Pages 880-886
نویسندگان
Shuang-Yan Yang, Fu-Lan Wei, Li-Hua Hu, Chun-Ling Wang,