Keywords: Microbial carbon; Microbial nitrogen; Microbial phosphorus; PLFA; qPCR; Epifluorescent microscopy; Arctic birch hummock tundra; Seasonal; Fungi; Bacteria;
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Keywords: Lawsonia intracellularis; Ileitis; Porcine circovirus type 2 (PCV2); Pigs; qPCR; ELISA; Faecal load; Antibody; Average daily gain (ADG);
Keywords: SHIME; simulator of the intestinal microbial ecosystem; DGGE; denaturing gradient gel electrophoresis; qPCR; quantitative PCR; SCFA; short chain fatty acid; RWGE; red wine grape extract; Antimicrobial; Gut; Microbiota; Polyphenol; SHIME;
Keywords: Impressed current; Clogging; Biofilm; qPCR; DGGE;
Keywords: b-SO6; biotinylated saporin-6; BCA; bicinchoninic acid; BFU-e; burst forming unit-erythroid; BHLHB2; basic-helix-loop-helix transcription factor B2; BMMC; bone marrow mononuclear cells; CDC14B; dual specificity protein tyrosine phosphatase family; CFU-e;
Keywords: Single cell; Gene expression; T-cell activation; qPCR;
Keywords: Prostate cancer; Chronic fatigue syndrome; Fatigue; qPCR; Gene expression; Purinergic; GABA-receptor modulator; Cytokine;
Keywords: ANA; anti-nuclear antibody; ATA; anti-topoisomerase antibody; AMA; anti-mitochondrial antibody; AAV; ANCA associated vasculitis; AFA; anti-fibroblast antibody; AECA; anti-endothelial cell antibody; anti-CCP; anti-cyclic citrullinated peptide; anti-CENP; a
Keywords: Cigarette smoke; chronic obstructive pulmonary disease; inflammation; emphysema; airway remodeling; lung function; macrophage; mast cell; protease; mouse mast cell protease 6; htryptase-β; B6; C57BL/6; BALF; Bronchoalveolar lavage fluid; COPD; Chronic ob
Keywords: POCT; Bacterial sepsis detection; Blood; Magnetic nanoparticles; qPCR;
Keywords: Digital PCR; Copy number estimation; Serial dilution; Poisson distribution; Virus; Maximum likelihood; dPCR; digital polymerase chain reaction; qPCR; quantitative real-time PCR; RT-qPCR; reverse transcription-qPCR; MLE; maximum-likelihood estimation; SE;
Keywords: Limit of detection; Limit of quantification; LoD; LoQ; GenEx software; Real-time PCR; qPCR; Data analysis; Replicates; MIQE; Quality control; Standardization;
Keywords: Oenococcus oeni; Malolactic fermentation; Thioredoxin; Stress; qPCR
Keywords: Clubroot management; Brassica napus L. subsp. napus; Resistant cultivars; qPCR; Infested field
Keywords: Horse; Hindgut; Bacteria; Archaea; DGGE; Qpcr
Keywords: Eg5; LSCC; qPCR; IHC
Keywords: Research; Reproducibility; Biomedicine; qPCR; Microarrays; Next generation sequencing
Keywords: Freeze tolerance; Metal toxicity; Metallothionein; Oxidative stress; qPCR
Keywords: Freeze-dried; Lyophilization; qPCR; RNA quality; RNA storage; RIN number
Keywords: RNA extraction and virus detection from seedsSMV; WSMV; Quarantine; Seed certification; RT-PCR; qPCR
Keywords: ERCC; Evaluation material; Interlaboratory study; Saccharomyces cerevisiae; qPCR; Reference material
Keywords: Composting; Flue gas desulphurization gypsum; Nitrification; Denitrification; qPCR
Keywords: Chromatin; qPCR; Benzonase
Keywords: Bioremediation; Rhodococcus erythropolis; Hydrocarbons (HC); Oxitop; qPCR; 16S-rRNA gene sequencing
Keywords: Mycoplasma; qPCR; Biopharmaceuticals; Quality control
Keywords: Forensic genetics; MicroRNA; qPCR; Body fluid identification
Angiopoietin-like 8 (ANGPTL8) expression is regulated by miR-143-3p in human hepatocytes
Keywords: ANGPTL8; Angiopoietin-like protein 8; HDL-C; High density lipoprotein cholesterol; 3'UTR; 3â² untranslated region; siRNA; Silencing RNA; LPS; Lipopolysaccharide; CAD; Coronary artery disease; LDL-C; Low-density lipoprotein cholesterol; miRNA; MicroRNA;
Microcystin-LR promotes migration via the cooperation between microRNA-221/PTEN and STAT3 signal pathway in colon cancer cell line DLD-1
Keywords: MC-LR; Microcystin-LR; PI3-K/AKT; Phosphatidylinositol 3-kinase/Akt; PP1 and PP2A; Protein phosphatase type 1 and type 2A; qPCR; Quantitative real-time polymerase chain reaction; STAT3; Signal transducer and activator of transcription 3; siRNA; Small inte
Expression and function of lncRNA MALAT-1 in the embryonic development of zebrafish
Keywords: AS; alternative splicing; B-MYB; Myb-related protein B; Egr1; early growth response 1; hpf; hour post-fertilization; lncRNA; long non-coding RNA; MALAT-1; metastasis-associated lung adenocarcinoma transcript 1; mascRNA; MALAT-1-associated small cytoplasmi
Quantitative PCR coupled with melting curve analysis for rapid detection and quantification of Tenacibaculum maritimum in fish and environmental samples
Keywords: Tenacibaculum maritimum; Tenacibaculosis; Diagnosis; qPCR; Melting curve;
Impact of origin (wild vs. farmed) and sea lice (Lepeophtheirus salmonis) infestation on expression of immune-relevant genes in Atlantic salmon (Salmo salar L.) skin
Keywords: Atlantic salmon; Lepeophtheirus salmonis; wild-farmed interaction; qPCR; immunology;
Molecular cloning, characterization and expression analysis of ARMC6, ARMC7, ARMC8 from Pacific white shrimp, Litopenaeus vannamei
Keywords: ARMC; armadillo repeat-containing protein; ARM; armadillo; ORF; open reading frames; WSSV; white spot syndrome virus; qPCR; real-time quantitative PCR; hpi; hour post-injection; NCBI; Biotechnology information databases; SEM; standard error of the mean; L
Histopathological and novel high-throughput molecular monitoring data from farmed salmon (Salmo salar and Oncorhynchus spp.) in British Columbia, Canada, from 2011-2013
Keywords: Aquaculture; Atlantic salmon; Pacific salmon; Histopathology; qPCR; APC; (artificial positive clone); AS; (Atlantic salmon); BC; (British Columbia); CFIA; (Canadian Food Inspection Agency); CI; (confidence interval); CNS; (central nervous system); Ct; (cy
A pilot program for clinical Q fever surveillance as a first step for a standardized differential diagnosis of abortions: Organizational lessons applied to goats farms
Keywords: Q fever; Abortions; Goats; Surveillance; Standardized diagnosis; qPCR;
All iguana families with the exception of basilisks share sex chromosomes
Keywords: Dedifferentiation; Sex chromosomes; Evolution; qPCR; X-linked genes;
Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans
Keywords: BTF; biotrickling filter; cin; inlet TMA concentration; cout; outlet TMA concentration; DGGE; denaturing gradient gel electrophoresis; DMA; diethylamine; EBRT; empty bed residence time; EC; elimination capacity (gTMA·m-3·h-1); F; flow (m3·h-1); H; heig
Utilization of real time PCR for the assessment of egg burden in the organs of Schistosoma japonicum experimentally infected mice
Keywords: qPCR; Real time PCR; Schistosomiasis; S. japonicum;
Flow cytometry as a new complementary tool to study Coxiella burnetii in cell cultures
Keywords: Coxiella bunetii; Cell culture; Flow cytometry; qPCR;
Using RNA-Seq with 11 marker genes to evaluate 1,4-dioxane compared with typical genotoxic and non-genotoxic rat hepatocarcinogens
Keywords: AAF; 2-acetoamidofluorene; DEHP; di(2-ethylhexyl)phthalate; DEN; N-nitrosodiethylamine; DMB; 33â²-dimethylbenzidine·2HCl; DO; 14-dioxane; GTHC; genotoxic hepatocarcinogen; IARC; International Agency for Research on Cancer; NGTHC; non-genotoxic hepatocar
Impact of various killing methods on EMA/PMA-qPCR efficacy
Keywords: Thermotolerant Campylobacter spp.; EMA; PMA; Killing assay; Viability determination; qPCR;
Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development
Keywords: CRB3; PALS1; PATJ; MPDZ; Mouse preimplantation embryo; TJ; tight junction; qPCR; Real-time quantitative PCR; TE; trophectoderm; HCG; human chorionic gonadotropin;
Producing standard damaged DNA samples by heating: pitfalls and suggestions
Keywords: qPCR; Degraded samples; DNA quantification; DNA hydrolysis;
The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients
Keywords: Cell-free fDNA; NanoDrop; Spectrophotometry; Fluorimetry; Qubit; qPCR; Malignant melanoma; Prostate cancer; Laboratory biomarkers; Circulating free tumor DNA; Tumor marker;
TGF-β autocrine signaling at secretory-stage enamel
Keywords: ALP; alkaline phosphatase; BMP; bone morphogenic protein; ELISA; enzyme-linked immunosorbent assay; HPLC; high performance liquid chromatography; MMP; matrix metalloproteinase; qPCR; quantitative polymerase chain reaction; RT-PCR; reverse transcription-po
Molecular characterization of interspecific competition of Diachasmimorpha longicaudata (Ashmead) and Fopius arisanus (Sonan) parasitizing the oriental fruit fly, Bactrocera dorsalis (Hendel)
Keywords: qPCR; Parasitism; Diachasmimorpha longicaudata (Ashmead); Fopius arisanus (Sonan); Bactrocera dorsalis (Hendel); Interspecific competition;
Development and validation of a quantitative PCR assay for detection of Emydoidea herpesvirus 1 in free-ranging Blanding's turtles (Emydoidea blandingii)
Keywords: Blanding's turtle; Chelonian; Emydoidea blandingii; Epidemiology; Herpesvirus; qPCR;
Evaluation of murine lung epithelial cells (TC-1 JHU-1) line to develop Th2-promoting cytokines IL-25/IL-33/TSLP and genes Tlr2/Tlr4 in response to Aspergillus fumigatus
Keywords: Aspergillus fumigatus; Epithelia; QPCR; IL-25; IL-33; TSLP; Tlr;
Association and expression analysis of single nucleotide polymorphisms of CD14 gene with somatic cell score in crossbred cattle
Keywords: CD14; Gene; In silico analysis; Polymorphism; qPCR; SCS;
Associations of GBP2 gene copy number variations with growth traits and transcriptional expression in Chinese cattle
Keywords: CNVs; Copy number variations; AJ; Angusâ¯Ãâ¯Jiaxian cattle; CDM; Chaidamu cattle; CJ; Charlotteâ¯Ãâ¯Jiaxian; FoSTeS; Fork Stalling and Template Switching; GBP2; guanylate binding protein 2; GWAS; genome-wide association study; JA; Jian cattle; JJ; J
Molecular characterisation of Salmonella enterica serovar Typhimurium and Campylobacter jejuni faecal carriage by captured rangeland goats
Keywords: qPCR; Salmonella; Campylobacter jejuni; Goat; Public health; Zoonotic;