An N-targeting real-time PCR strategy for the accurate detection of spring viremia of carp virus
Keywords: SVCV; Diagnosis; The N gene; RT-qPCR;
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Production of infectious dromedary camel hepatitis E virus by a reverse genetic system: Potential for zoonotic infection
Keywords: DcHEV; dromedary camel hepatitis E virus; ORF; open reading frame; VLPs; virus-like particles; G; genotype; DcHEV-LPs; virus-like particles of DcHEV; ELISA; enzyme-liked immunosorbent assay; OD; optical density; RT-qPCR; real-time quantitative polymerase
Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
Keywords: IBV; infectious bronchitis virus; CMI; cell-mediated immunity; CTL; cytotoxic T lymphocyte; MHC; major histocompatibility complex; SPF; specific-pathogen free; ELD50; chicken embryo lethal dose; EID50; median embryo infectious dose; ATCC; American Type Cu
USP15 stabilizes the transcription factor Nrf1 in the nucleus, promoting the proteasome gene expression
Keywords: Ubiquitination; Deubiquitination; Proteasomal degradation; Transcription factor; Gene regulation; ARE; antioxidant response element; CNC; Cap'n'Collar; DUB; deubiquitinating enzyme; ER; endoplasmic reticulum; NHB1; N-terminal homology box 1; Nrf1; NF-
Identification and validation of reference genes for quantitative real-time PCR studies in Hedera helix L.
Keywords: Reference gene; Hedera helix L.; RT-qPCR; Differential expression; Abiotic stress;
Cold stress affects H+-ATPase and phospholipase D activity in Arabidopsis
Keywords: 14-3-3 protein; Arabidopsis thaliana; Cold stress; H+-ATPase; Phosphatidic acid; Phospholipase D; AHA1; Arabidopsis thaliana H+-ATPase isoform 1; AHA2; Arabidopsis thaliana H+-ATPase isoform 2; CBF; C-repeat binding factor; DAG; diacylglycerol; FC; fusico
Iron deficiency resistance mechanisms enlightened by gene expression analysis in Paenibacillus riograndensis SBR5
Keywords: Iron deficiency; Paenibacillus genus; RNA seq; RT-qPCR;
Development and evaluation of a reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay for detection of equine arteritis virus in equine semen and tissue samples using the POCKIT⢠system
Keywords: EVA; equine viral arteritis; EAV; equine arteritis virus; VNT; virus neutralization test; OIE; World Organisation for Animal Health; ORF; open reading frame; RT-iiPCR; reverse transcription-insulated isothermal PCR; RT-qPCR; real-time RT-PCR; PFU; plaque-
A differential gene expression study: Ptpn6 (SHP-1)-insufficiency leads to neutrophilic dermatosis-like disease (NDLD) in mice
Keywords: Ptpn6; tyrosine phosphatase, non-receptor type-6; Hcph; hematopoietic cell phosphatase; Shp-1; Src homology 2 domain-containing tyrosine phosphatase 1; ITIM; immunoreceptor tyrosine-based inhibitory motif; ITAM; immunoreceptor tyrosine-based activation mo
Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri
Keywords: Seagrass; Molecular physiology; Isotope; Nutrients; Nitrogen; RT-qPCR;
Ovarian-like differentiation in eutopic and ectopic endometrioses with aberrant FSH receptor, INSL3 and GATA4/6 expression
Keywords: CYP11A1; Cytochrome P450 Family 11 Subfamily A Member 1; CYP17; Cytochrome P450 Family 17 Subfamily A Member 1; CYP19A1; Cytochrome P450 Family 19 Subfamily A Member 1; EEC; Epithelial Endometriotic Cells; EGVEGF; Endocrine Gland-derived vascular endothel
Neonatal ventral hippocampus lesion changes nuclear restricted protein/brain (NRP/B) expression in hippocampus, cortex and striatum in developmental periods of rats
Keywords: E18; embryonic day 18; NRF2; Nuclear Factor-related 2; NRP/B; nuclear restricted protein/brain; NS; normal saline; NVHL; neonatal ventral hippocampal lesions; PD; postnatal day; RT-qPCR; real-time quantitative PCR; TBST; Tween/Tris-buffered saline; schizo
Culicoides species composition and environmental factors influencing African horse sickness distribution at three sites in Namibia
Keywords: RT-qPCR; Culicoides imicola; Precipitation; Temperature;
A systematic review of human norovirus survival reveals a greater persistence of human norovirus RT-qPCR signals compared to those of cultivable surrogate viruses
Keywords: Systematic review; Norovirus; RT-qPCR; Survival; Persistence; Heat; Chlorine
Transcriptomic analysis of responses to cytopathic bovine viral diarrhea virus-1 (BVDV-1) infection in MDBK cells
Keywords: RT-qPCR; Meta-transcriptomic; BVDV-1; Flaviviridae
A role for mRNA trafficking and localized translation in peroxisome biogenesis and function?
Keywords: ChIP; chromatin immunoprecipitation; CLIP; crosslinking immunoprecipitation; ER; endoplasmic reticulum; FISH; fluorescent in situ hybridization; GET; Golgi-ER trafficking; ISH; in situ hybridization; MFP; multifunctional protein; mPP; mRNA encoding peroxi
Fe reduced hepatic lipid deposition in Synechogobius hasta exposed to waterborne Cu
Keywords: ACC; acetyl-CoA carboxylase; ATGL; adipose triglyceride lipase; ANOVA; one-way analysis of variance; CPT; carnitine palmitoyltransferase; Cu; copper; DG; diacylglycerol; FA; fatty acid; FAS; fatty acid synthase; Fe; iron; G6PD; glucose-6-phosphate dehydro
Gene expression profiling and DNA methylation analyses of CTCs
Keywords: Circulating Tumor Cells; CTCs; Molecular assays; Multiplex RT-PCR; Molecular characterization; Quantitative PCR (qPCR); Reverse transcription PCR; RT-qPCR; Methylation specific PCR; Gene expression; aCGH; array-comparative genomic hybridization; BRMS1; Br
A Transcriptomic Biomarker to Quantify Systemic Inflammation in Sepsis — A Prospective Multicenter Phase II Diagnostic Study
Keywords: Host response; Transcriptomic profiling; Adaptive immunity; Point-of-care; RT-qPCR; Clinical utility
Presence and characterization of pig group A and C rotaviruses in feces of Belgian diarrheic suckling piglets
Keywords: Group A rotavirus; Group C rotavirus; Pig; Diarrhea; RT-qPCR; Bacteria
Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic
Keywords: AOI; area of interest; BK; breaker; CDK; cyclin-dependent kinase; DPA; days post-anthesis; DPS; days post-sowing; GRF; growth-regulating factor; MC; MACROCALYX; MG; mature green; miRNA; microRNA; MT; Micro-Tom; NCBI; National Center for Biotechnology Info
A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts
Keywords: cardiac fibroblasts; collagen; histone methylation; transcriptome; aHCF; adult human cardiac fibroblast; ATAC; assay for transposase accessible chromatin; ATAC-seq; assay for transposase accessible chromatin-sequencing; CF; cardiac fibroblast; ChIP-seq;
Reference genes for valid gene expression studies on rat dental, periodontal and alveolar bone tissue by means of RT-qPCR with a focus on orthodontic tooth movement and periodontitis
Keywords: RT-qPCR; Gene expression; Gene expression regulation; Rats, Inbred F344; Orthodontics; Periodontitis; Dental research;
Comparative transcriptomics reveals molecular components associated with differential lipid accumulation between microalgal sp., Scenedesmus dimorphus and Scenedesmus quadricauda
Keywords: CDS; Coding Sequence; GO; Gene Ontology; KO; KAAS Orthology; KAAS; KEGG Automatic Annotation Server; KEGG; Kyoto Encyclopedia of Genes and Genomes; RSEM; RNA-Seq by Expectation Maximization; TPM; Transcript Per Million; FPKM; Fragments Per Kilobase per Mi
An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1
Keywords: RNA copy number ratio; RT-qPCR; Gene expression; Normalisation; Standardisation; Molecular diagnostic; Transcriptomics; Cancer; Diagnostics; Biomarker identification and validation
Molecular and functional characterization of Toll-like receptor (Tlr)1 and Tlr2 in common carp (Cyprinus carpio)
Keywords: Toll-like receptor; Innate immunity; Heterodimer; TLR signalling; Teleost; BSA; bovine serum albumin; GFP; green fluorescent protein; IRAK1; interleukin-1 receptor-associated kinase 1; LRR; leucine-rich repeat; LTA; lipoteichoic acid; MYD88; myeloid diffe
Molecular characterization and expression analyses of three RIG-I-like receptor signaling pathway genes (MDA5, LGP2 and MAVS) in Larimichthys crocea
Keywords: Large yellow croaker; Innate antiviral immunity; Pattern recognition receptor; Poly (I:C); RT-qPCR
Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus
Keywords: Infectious bronchitis; IBV; RT-qPCR; Genotype;
Identification of reference genes for quantitative RT-PCR analysis of microRNAs and mRNAs in castor bean (Ricinus communis L.) under drought stress
Keywords: Drought; Internal control genes; Ricinus communis L.; RT-qPCR; Transcript normalization;
Activation of AIFM2 enhances apoptosis of human lung cancer cells undergoing toxicological stress
Keywords: DDP; cisplatin; JFK; Jinfukang; TCM; Traditional Chinese Medicine; RNA; seq RNA sequencing; CCK8; cell counting kit 8; PI; propidium iodine; RT-qPCR; quantitative real-time PCR; DAVID; database for annotation visualization and integrated discovery; GO; ge
Molecular cloning and expression of a novel eukaryotes elongation factor1A gene (ZjeEF-1α) from Chinese jujube in response to phytoplasma infection
Keywords: ZjeEF-1α; Chinese jujube; Phytoplasma; RT-qPCR; Western blotting;
Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus
Keywords: Virus; Carrots; RT-PCR; RT-qPCR;
Role of miR-181a-5p and endoplasmic reticulum stress in the regulation of myogenic differentiation
Keywords: ATF6; activating transcription factor 6; BMP4; bone morphogenetic protein 4; DM; differentiation medium; ER; endoplasmic reticulum; GM; growth medium; GRP78/BIP; glucose-regulated protein of 78Â kDa/binding protein for immunoglobulins; IGF-1; insulin-like
Dissecting the Roles of Polycomb Repressive Complex 2 Subunits in the Control of Skin Development
Keywords: FACS; fluorescence-activated cell-sorting; Krt; keratin; PRC2; polycomb repressive complex 2; RT-qPCR; semiquantitative real-time PCR;
Boron stress response and accumulation potential of the extremely tolerant species Puccinellia frigida
Keywords: Bshoots; boron concentration in shoots; Bsupply; boron supply concentration; Broots; boron concentration in roots; BF; bioconcentration factor; DW; dry weight; EC50; effective concentration 50%; EST; expressed sequence tag; FW; fresh weight; LC50; lethal
Quantitative real-time PCR normalization for gene expression studies in the plant pathogenic fungi Lasiodiplodia theobromae
Keywords: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; ACT; actin; TUB; β-tubulin; EF1α; elongation factor-1α; RT-qPCR; reverse transcription polymerase chain reaction; RNAseq; RNA sequencing; qPCR; quantitative PCR; gDNA; genomic DNA; cDNA; complementary; T
Molecular characterization and functional analysis of BdFoxO gene in the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)
Keywords: TOR; target of rapamycin; JNK; Jun NH2-terminal kinase; FoxO; forkhead box O; InR; insulin receptor; Akt; protein kinase B; PI3K; phosphatidylinositol 3-kinase; 4EBP; 4E binding protein; ILPs; insulin-like peptides; RT-qPCR; reverse transcription-quantita
Effect of the addition of phytosterols and tocopherols on Streptococcus thermophilus robustness during industrial manufacture and ripening of a functional cheese as evaluated by qPCR and RT-qPCR
Keywords: AHA; American Heart Association; C; control; Ct; threshold cycle; Cu; curd; C7d; C cheese ripened for 7 days at 4 °C; F; functional; FAMEs; fatty acid methyl esters; F7d; F cheese ripened for 7 days at 4 °C; HPLC; high-pressure liquid chromatography
Superparamagnetic iron oxide nanoparticle uptake alters M2 macrophage phenotype, iron metabolism, migration and invasion
Keywords: AD; Aminodextran; APS; 3-aminopropyl-triethoxysilane; BMM; Bone marrow-derived macrophages; BMM-M2; Bone marrow-derived macrophages polarised to M2; DMSA; Dimercaptosuccinic acid; DMT-1; Divalent metal transporter-1; ERK; Extracellular signal-regulated ki
Evaluating the influence of light intensity in mcyA gene expression and microcystin production in toxic strains of Planktothrix agardhii and Microcystis aeruginosa
Keywords: Planktothrix agardhii; Microcystis aeruginosa; Light intensity; Microcystin; mcyA gene; RT-qPCR;
Novel non-peptide small molecules preventing IKKβ/NEMO association inhibit NF-κB activation in LPS-stimulated J774 macrophages
Keywords: ADME; absorption, distribution, metabolism, and excretion; DMEM; Dulbecco's modified Eagle's medium; DMSO; dimethyl sulfoxide; EMSA; Electrophoretic Mobility Shift Assay; Erk; extracellular-signal-related kinase; FBS; foetal bovine serum; GAPDH; glyce
N-glycosylation-mutated HCV envelope glycoprotein complex enhances antigen-presenting activity and cellular and neutralizing antibody responses
Keywords: Antigen presenting cell; APC; cell culture-derived HCV; HCVcc; Cytotoxic T lymphocyte; CTL; enzyme-linked immunospot assay; ELISPOT; half-maximal effective concentration; EC50; Hepatitis C Virus; HCV; HCV pseudo particles; HCVpp; interleukin; IL; interfer
Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica)
Keywords: Lamprey; Buccal gland; CRBGP; Anti-angiogenesis; APs; action potentials; BCA; bicinchoninic acid; bFGF; basic fibroblast growth factor; Ca2+; calcium; CAM; chick chorioallantoic membrane; CNG; cyclic nucleotide-gated ion; CRBGP; cysteine-rich buccal gland
Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
Keywords: Freeze-dried; RNA integrity number; RT-qPCR; Vitis vinifera L; Woody tissue
Rapid RNA analysis of individual Caenorhabditis elegans
Keywords: Rapid single worm RT-qPCRC. elegans; RNA; RT-qPCR
Quantification of EVI1 transcript levels in acute myeloid leukemia by RT-qPCR analysis: A study by the ALFA Group
Keywords: EVI1 overexpression; Acute myeloid leukemia; RT-qPCR; MLL
Soluble ST2 and IL-33: Potential markers of endometriosis in the Tunisian population
Keywords: Inflammation; IL-33; Soluble ST2; Endometriosis; RT-qPCR; ELISAIL-33, interleukin-33; ST2, Th2-specific molecule; PBMC, peripheral blood mononuclear cells; ELISA, sandwich enzyme-linked immunosorbent assay; PF, peritoneal fluid; RT-qPCR, real-time reverse
Very low-dose (femtomolar) 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) disrupts steroidogenic enzyme mRNAs and steroid secretion by human luteinizing granulosa cells
Keywords: Dioxin; Estrogen; Granulosa cells; Low dose; Ovary; RT-qPCR;
Reduced SIRT1 expression correlates with enhanced oxidative stress in compensated and decompensated heart failure
Keywords: Sirtuin; RT-qPCR; TAS; TOS; OSI; HDL
Effects of copper-oxide nanoparticles, dissolved copper and ultraviolet radiation on copper bioaccumulation, photosynthesis and oxidative stress in the aquatic macrophyte Elodea nuttallii
Keywords: Macrophyte; CuO-nanoparticles; Accumulation; Oxidative stress; Superoxide dismutase; RT-qPCR