کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905084 | 1159829 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of miR-181a-5p and endoplasmic reticulum stress in the regulation of myogenic differentiation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Thapsigarginmyogenic regulatory factor 4myogenic differentiation antigeninositol-requiring enzyme 1 alphaMrf4Myf5MyogeninMyoDMyoRtunicamycinATF6UPRBMP4IGF-1NF-κBmiRNAsRT-qPCRPMFGRP78/BiP - GRP78 / BIPIRE1α - IRE1aROS - ROSEndoplasmic reticulum stress - استرس شبکه آندوپلاسمیdifferentiation medium - تمایز رسانهMyogenic differentiation - تمایز میوگنApoptosis - خزان یاختهایmicroRNAs - ریز آرانایendoplasmic reticulum - شبکه آندوپلاسمی Myogenic Factor 5 - عامل فاکتور 5Insulin-like growth factor - فاکتور رشد مانند انسولینnuclear factor κB - فاکتور هسته ای κBactivating transcription factor 6 - فعال کردن عامل رونویسی 6Growth medium - محیط رشدSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیMYOG - منوreal-time quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استUnfolded protein response - پاسخ پروتئین آشکارbone morphogenetic protein 4 - پروتئین مورفوژنیک استخوان 4PERK - پرکReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Accumulating evidence has indicated that microRNAs (miRNAs) and endoplasmic reticulum (ER) stress play critical roles in myoblast differentiation. However, the regulation roles of miRNAs and ER stress in myogenic differentiation have not been fully revealed and need to be further studied. Here, we discovered that the expression levels of miR-181a-5p were strongly upregulated during C2C12 cell differentiation. miR-181a-5p overexpression promoted ER stress and differentiation of C2C12 cells, which was accompanied by increasing expression levels of marker genes related to ER stress-mediated apoptosis and myogenic differentiation. Opposite results were observed after inhibition of the miR-181a-5p expression. The gain- and loss-of-function experiments on C2C12 cells showed that miR-181a-5p affected the development of muscle fiber type, but had no significant influence on C2C12 cell proliferation. In the ER-stressed C2C12 cells induced by thapsigargin (Tg), the expression levels of both miR-181a-5p and marker genes related to ER stress and myogenesis were upregulated. In the ER-stressed C2C12 cells and porcine muscle fibroblast (PMF) cells pretreated with Tg, we found that miR-181a-5p targeted glucose-regulated protein, 78Â kDa/binding immunoglobulin protein (GRP78/BIP), and influenced cell apoptosis. In conclusion, these results indicate that miR-181a-5p and ER stress have positive synergistic effects on myogenic differentiation by increasing the expression levels of myogenic differentiation key genes and activating the ER stress-mediated apoptosis signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 592, Issue 1, 30 October 2016, Pages 60-70
Journal: Gene - Volume 592, Issue 1, 30 October 2016, Pages 60-70
نویسندگان
Yingying Wei, Xuelian Tao, Huaming Xu, Yan Chen, Li Zhu, Guoqing Tang, Mingzhou Li, Anan Jiang, Surong Shuai, Jideng Ma, Long Jin, Anxiang Wen, Qin Wang, Guangxiang Zhu, Meng Xie, Jiayun Wu, Tao He, Yanzhi Jiang, Xuewei Li,