دانلود مقالات ISI درباره mutagenesis مواجه با سایت + ترجمه فارسی

Site-directed mutagenesis around the CuA site of a polyphenol oxidase from Coreopsis grandiflora (cgAUS1)

Keywords: mutagenesis مواجه با سایت; AAS; atomic absorption spectroscopy; AmAS1; aureusidin synthase from Antirrhinum majus (protein); aoTYR; tyrosinase from Aspergillus oryzae (protein); bmTYR; tyrosinase from Bacillus megaterium (protein); cgAUS1; aurone synthase from Coreopsis grandiflora

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2015 سفارش ترجمه

Probing the chemical mechanism of saccharopine reductase from Saccharomyces cerevisiae using site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Saccharopine reductase; Site-directed mutagenesis; Initial rate studies; pH-rate profiles; Isotope effects; ViscosityAAA, α-aminoadipate; SR, saccharopine reductase (saccharopine dehydrogenase: l-glutamate forming); NADP, nicotinamide adenine dinucleotide

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2015 سفارش ترجمه

Functional elements of the gastric inhibitory polypeptide receptor: Comparison between secretin- and rhodopsin-like G protein-coupled receptors

Keywords: mutagenesis مواجه با سایت; G protein-coupled receptor (GPCR); G protein; Molecular modeling; Homology modeling; Site-directed mutagenesis; Secretin-like receptor; Gastric glucose-dependent insulinotropic polypeptide receptor (GIPR);

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2015 سفارش ترجمه

A catalytic triad - Lys-Asn-Asp - Is essential for the catalysis of the methyl transfer in plant cation-dependent O-methyltransferases

Keywords: mutagenesis مواجه با سایت; SAM; S-adenosyl-l-methionine; CCoAOMT; caffeoyl-coenzyme A dependent O-methyltransferase; OMT; O-methyltransferase; queg; quercetagetin; Ice plant; Mesembryanthemum crystallinum; Aizoaceae; Methyltransferase; Catalytic triad; Molecular modelling; Site-dir

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2015 سفارش ترجمه

Active site cleft mutants of Os9BGlu31 transglucosidase modify acceptor substrate specificity and allow production of multiple kaempferol glycosides

Keywords: mutagenesis مواجه با سایت; Rice (Oryza sativa L.); Transglucosidase; Site-directed mutagenesis; Glycoside hydrolase family GH1; Glycosylation; Electrospray ionization mass spectrometry;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2015 سفارش ترجمه

Identification of three important amino acid residues of xylanase AfxynA from Aspergillus fumigatus for enzyme activity and formation of xylobiose as the major product

Keywords: mutagenesis مواجه با سایت; Xylanase; Xylobiose; Aspergillus fumigatus; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2015 سفارش ترجمه

A salt-bridge stabilized C-terminal hook is critical for the dimerization of a Bowman Birk inhibitor

Keywords: mutagenesis مواجه با سایت; Knob-in-the-hole; Site-directed mutagenesis; Molecular dynamics simulations; Salt bridge; Substrate-induced monomerisation; Trypsin/chymotrypsin inhibitor

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2015 سفارش ترجمه

Predicting a double mutant in the twilight zone of low homology modeling for the skeletal muscle voltage-gated sodium channel subunit beta-1 (Nav1.4 β1)

Keywords: mutagenesis مواجه با سایت; MD-2, myeloid differentiation factor 2 (MD-2); SDM, site-directed mutagenesis; Nav1.4, skeletal muscle voltage-gated sodium channel; TLR4, Toll-like receptor type 4; Trk, tyrosine receptor.Ig-like; CDR1; MD-2; Patch-clamp; Site-directed mutagenesis; Analo

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2015 سفارش ترجمه

Hitting the right spot: Mechanism of action of OPB-31121, a novel and potent inhibitor of the Signal Transducer and Activator of Transcription 3 (STAT3)

Keywords: mutagenesis مواجه با سایت; STAT3; Transcription factors; Small molecule inhibitors; Anticancer drugs; Cancer therapy; Mechanism of action; Computational modeling; Molecular dynamic simulation; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2015 سفارش ترجمه

Interactions between permeant and blocking anions inside the CFTR chloride channel pore

Keywords: mutagenesis مواجه با سایت; Chloride channel; Cystic fibrosis transmembrane conductance regulator; Ion binding; Multi-ion pore behavior; Open channel block; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 18 صفحه سال انتشار : 2015 سفارش ترجمه

Modulation of transglycosylation and improved malto-oligosaccharide synthesis by protein engineering of maltogenic amylase from Bacillus lehensis G1

Keywords: mutagenesis مواجه با سایت; Maltogenic amylase; Transglycosylation; Malto-oligosaccharide; Site-directed mutagenesis; Protein engineering;

دانلود رایگان متن کامل مقاله ISI 40 صفحه سال انتشار : 2015 سفارش ترجمه

Substitution of Thr55 by Gly and Lys48 by Asp in OsTrx20 using site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Rice; Site-directed mutagenesis; Thioredoxin; OsTrx20IPTG, isopropyl β-d-thiogalactopyranoside; Trx, Thioredoxin; Os, Oryza sativa

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2014 سفارش ترجمه

Effect of surface charge alteration on stability of l-asparaginase II from Escherichia sp.

Keywords: mutagenesis مواجه با سایت; l-Asparaginase; Site-directed mutagenesis; Surface charge alteration; Thermal tolerance

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2014 سفارش ترجمه

Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants

Keywords: mutagenesis مواجه با سایت; Enzyme activity; Circular dichroism; Glucose-1-dehydrogenase; Low-temperature activity; Site-directed mutagenesis; Steady-state kinetics; Thermal stability; Thermophilic enzyme

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2014 سفارش ترجمه

Mechanism-based site-directed mutagenesis to shift the optimum pH of the phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1

Keywords: mutagenesis مواجه با سایت; Phenylalanine ammonia-lyase; Rhodotorula glutinis JN-1; Friedel–Crafts-type mechanism; Site-directed mutagenesis; Resolution; The optimum pH

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Improved thermostability of esterase from Aspergillus fumigatus by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Esterase; Site-directed mutagenesis; Thermostability; Half-life

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Phosphorylation of sterol regulatory element binding protein-1a by protein kinase A (PKA) regulates transcriptional activity

Keywords: mutagenesis مواجه با سایت; Sterol regulatory element binding protein (SREBP); Protein kinase A (PKA); Immobilized metal affinity chromatography (IMAC); Phosphorylation; Site-directed mutagenesis; Mass spectrometry (MS);

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Examining the critical roles of human CB2 receptor residues Valine 3.32 (113) and Leucine 5.41 (192) in ligand recognition and downstream signaling activities

Keywords: mutagenesis مواجه با سایت; Molecular modeling; Site-directed mutagenesis; Cannabinoid receptor subtype 2 (CB2); Cyclic adenosine monophosphate (cAMP); Time-resolved fluorescence resonance energy (TR-FRET) transfer; Adenylyl cyclase (AC activity);

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Improved catalytic efficiency of catalase from Bacillus subtilis by rational mutation of Lys114

Keywords: mutagenesis مواجه با سایت; Catalase; Enzyme engineering; Site-directed mutagenesis; Catalytic efficiency

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2014 سفارش ترجمه

Characterization and site-directed mutagenesis of an α-galactosidase from the deep-sea bacterium Bacillus megaterium

Keywords: mutagenesis مواجه با سایت; α-Galactosidase; Glycoside hydrolase family 4; NAD+ binding; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Insights in the kinetic mechanism of the eukaryotic Baeyer-Villiger monooxygenase BVMOAf1 from Aspergillus fumigatus Af293

Keywords: mutagenesis مواجه با سایت; Baeyer-Villiger monooxygenases; BVMOAf1; Aspergillus fumigatus; Kinetic mechanism; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092

Keywords: mutagenesis مواجه با سایت; Maltooligosyltrehalose synthase; Hydrogen bond; Site-directed mutagenesis; Selectivity; Computer simulation; Sulfolobus

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Probing the ubiquinol-binding site of recombinant Sauromatum guttatum alternative oxidase expressed in E. coli membranes through site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Q(H2); (reduced) ubiquinone; OG; octyl gallate; SHAM; salicylic hydroxamic acid; CB; colletochlorin B; Asco; ascofuranone; AOX; alternative oxidase; rSgAOX; recombinant Sauromatum guttatum alternative oxidase; Alternative oxidase; Quinol oxidase; Oxygen a

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Expression of recombinant human IL-4 in Pichia pastoris and relationship between its glycosylation and biological activity

Keywords: mutagenesis مواجه با سایت; N-glycosylation; Site-directed mutagenesis; Recombinant human IL4; Pichia pastoris;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Enhancement of α-cyclodextrin product specificity by enriching histidines of α-cyclodextrin glucanotransferase at remote subsite −6

Keywords: mutagenesis مواجه با سایت; α-Cyclodextrin glucanotransferases; α-Cyclodextrin; Product specificity; Site-directed mutagenesis; Enzymatic properties

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate

Keywords: mutagenesis مواجه با سایت; Polyhydroxyalkanoates; Class II PHA synthase; Saturation mutagenesis; Site-directed mutagenesis; Substrate specificity

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Characterization and structure basis of Pseudomonas alcaligenes lipase's enantiopreference towards d,l-menthyl propionate

Keywords: mutagenesis مواجه با سایت; PaL; Enantiopreference; l-menthol; Molecular dynamics simulation; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Dissecting the catalytic and substrate binding activity of a class II lanthipeptide synthetase BovM

Keywords: mutagenesis مواجه با سایت; Lanthipeptide synthetase; Site-directed mutagenesis; Dehydration; Substrate binding;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Introducing transglycosylation activity in Bacillus licheniformis α-amylase by replacement of His235 with Glu

Keywords: mutagenesis مواجه با سایت; Bacillus licheniformis thermostable α-amylase; Substrate transglycosylation; Site-directed mutagenesis; Transfer product; Binding-subsite mapping;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2014 سفارش ترجمه

Sustained photobiological hydrogen production in the presence of N2 by nitrogenase mutants of the heterocyst-forming cyanobacterium Anabaena

Keywords: mutagenesis مواجه با سایت; Cyanobacteria; Anabaena; Hydrogen production; Biofuel; Nitrogenase; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

Characterization of bacterial NMN deamidase as a Ser/Lys hydrolase expands diversity of serine amidohydrolases

Keywords: mutagenesis مواجه با سایت; NMN; nicotinamide mononucleotide; NaMN; nicotinic acid mononucleotide; NaAD; nicotinic acid adenine dinucleotide; HPLC; high pressure liquid chromatography; PMSF; phenylmethylsulfonylfluoride; CD; circular dichroism; NMN deamidase; Pyridine nucleotide; Ca

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

Functional characterization of a Plagiochasma appendiculatum flavone synthase I showing flavanone 2-hydroxylase activity

Keywords: mutagenesis مواجه با سایت; Liverworts; Flavone synthase; Flavonol synthase; 2-Hydroxyflavanone; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

Site-directed mutagenesis studies of the aromatic residues at the active site of a lipase from Malassezia globosa

Keywords: mutagenesis مواجه با سایت; Lipase; Site-directed mutagenesis; Catalytic pocket; Substrate selectivity; Thermostability; CDocker;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

γ-Glutamyl transpeptidase architecture: Effect of extra sequence deletion on autoprocessing, structure and stability of the protein from Bacillus licheniformis

Keywords: mutagenesis مواجه با سایت; Protein stability; γ-Glutamyl transpeptidase; Ntn hydrolase; Site-directed mutagenesis; Deletion mutants;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2014 سفارش ترجمه

Point mutations in firefly luciferase C-domain demonstrate its significance in green color of bioluminescence

Keywords: mutagenesis مواجه با سایت; Firefly luciferase; Bioluminescence; Color-tuning mechanism; Site-directed mutagenesis; Oxyluciferin;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2014 سفارش ترجمه

Involvement of Val 315 located in the C-terminal region of thermolysin in its expression in Escherichia coli and its thermal stability

Keywords: mutagenesis مواجه با سایت; CBB; Coomassie Brilliant Blue; CC; Correlation coefficient; CD; Circular dichroism; DA; Degree of activation; EA; Expression amount; FAGLA; N-[3-(2-Fury)acryloyl]-glycyl-l-leucine amide; HC; α-helix content; PU; Proteolytic unit; REA; Relative expression

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2014 سفارش ترجمه

Understanding carbamoyl phosphate synthetase (CPS1) deficiency by using the recombinantly purified human enzyme: Effects of CPS1 mutations that concentrate in a central domain of unknown function

Keywords: mutagenesis مواجه با سایت; Urea cycle diseases; CPS1 deficiency; Hyperammonemia; Inborn errors; CPS 1 structure; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2014 سفارش ترجمه

Structure-function studies on jaburetox, a recombinant insecticidal peptide derived from jack bean (Canavalia ensiformis) urease

Keywords: mutagenesis مواجه با سایت; Jbtx; jaburetox; JbtxΔ-β; β-hairpin deleted version of Jbtx; Jbtx N-ter; N-terminal domain of Jbtx; Jbtx C-ter; C-terminal domain of Jbtx; Jbtx-2Ec; a version of Jbtx containing a V5 epitope; LUV; large unilamellar vesicle; MD; molecular dynamics; RMSD

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2014 سفارش ترجمه

Role of glutamine-169 in the substrate recognition of human aminopeptidase B

Keywords: mutagenesis مواجه با سایت; APB; aminopeptidase B; IC50; half maximal (50%) inhibitory concentration; EC50; half maximal (50%) effective concentration; ERAP; endoplasmic reticulum aminopeptidase; LTA4H; leukotriene A4 hydrolase; MCA; 4-methylcoumaryl-7-amide; NRD; N-arginine dibasic

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2014 سفارش ترجمه

COFACTOR SPECIFICITY ENGINEERING OF STREPTOCOCCUS MUTANS NADH OXIDASE 2 FOR NAD(P)+ REGENERATION IN BIOCATALYTIC OXIDATIONS

Keywords: mutagenesis مواجه با سایت; coenzyme selectivity; NADPH recycling; 2-heptanol oxidation; NADPH oxidase; site-directed mutagenesis; cofactor regeneration

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2014 سفارش ترجمه

Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain

Keywords: mutagenesis مواجه با سایت; AML; acute myeloid leukemia; BRPF1; bromodomain-PHD finger protein 1; CBP; CREB binding protein; CD; circular dichroism; HAT; histone acetyltransferase; HDAC; histone deacetylase; hEAF6; homolog of Esa1-associated factor 6; HOX; homeobox; HSCs; hematopoie

دانلود رایگان متن کامل مقاله ISI 11 صفحه سال انتشار : 2014 سفارش ترجمه

The role of the high potential form of the cytochrome b559: Study of Thermosynechococcus elongatus mutants

Keywords: mutagenesis مواجه با سایت; B band; thermoluminescence emission due to S2/3QB− charge recombination reaction; Chl; chlorophyll; Cm; chloramphenicol; CP43; a proximal antenna complex of photosystem II; Cyt; cytochrome; Eh; redox potential of the solution; Em; midpoint redox potenti

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2014 سفارش ترجمه

Complete sequence of the suicide vector pJP5603

Keywords: mutagenesis مواجه با سایت; pJP5603; Suicide vector; Recombinant DNA; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2013 سفارش ترجمه

A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction

Keywords: mutagenesis مواجه با سایت; AnSh; Aspergillus niger salicylate hydroxylase; OE-PCR; over extension PCR; PCR; polymerase chain reaction; SDL; DNA splicing by directed ligation; SDM; site-directed mutagenesis; Class-IIS endonuclease; Direct full-length polymerase chain reaction (PCR);

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2013 سفارش ترجمه

Improvement of pBBR1MCS plasmids, a very useful series of broad-host-range cloning vectors

Keywords: mutagenesis مواجه با سایت; pBBR1MCS vectors; Site-directed mutagenesis; NdeI site; pBBR1MCS_START series;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2013 سفارش ترجمه

Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase

Keywords: mutagenesis مواجه با سایت; GSH; glutathione; GST; glutathione transferase; GSTO; Omega-class GST; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Crystal structure; Glutathione; Glutathione transferase; Lepidoptera; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Thermostability improvement of maltogenic amylase MAUS149 by error prone PCR

Keywords: mutagenesis مواجه با سایت; Error prone PCR; Site-directed mutagenesis; Maltogenic amylase; Thermostability; MAU149; maltogenic amylase US149; MA-A27; mutated maltogenic amylase of clone A27; CD; cyclodextrin;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Human α-N-acetylglucosaminidase: cDNA cryptic site removal and native secretion signal addition significantly enhance enzyme expression and secretion

Keywords: mutagenesis مواجه با سایت; MPS IIIB; mucopolysaccharidosis III subtype B or Sanfilippo syndrome type B; Naglu; human α-N-acetylglucosaminidase; Sf9; Spodoptera frugiperda; modTat; synthetically optimized protein transduction domain also known as PTD4; RT-PCR; reverse transcription

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Exploring a potential palonosetron allosteric binding site in the 5-HT3 receptor

Keywords: mutagenesis مواجه با سایت; 5-HT; 5-hydroxytryptamine (serotonin); EC50; concentration of agonist required for half-maximal response; HEK; human embryonic kidney; IC50; concentration of antagonist required for half-maximal inhibition; Kd; affinity constant; Serotonin receptor; Allos

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Agonists and antagonists induce different palonosetron dissociation rates in 5-HT3A and 5-HT3AB receptors

Keywords: mutagenesis مواجه با سایت; Serotonin receptor; Allosteric binding site; Site-directed mutagenesis; Radioligand binding; FlexStation assays; 5-HT; 5-hydroxytryptamine (serotonin); HEK; human embryonic kidney; IC50; concentration of antagonist required for half-maximal inhibition; Kd

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه