دانلود مقالات ISI درباره mutagenesis مواجه با سایت + ترجمه فارسی

Thermostability improvement of maltogenic amylase MAUS149 by error prone PCR

Keywords: mutagenesis مواجه با سایت; Error prone PCR; Site-directed mutagenesis; Maltogenic amylase; Thermostability; MAU149; maltogenic amylase US149; MA-A27; mutated maltogenic amylase of clone A27; CD; cyclodextrin;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Three-dimensional structure of a Bombyx mori Omega-class glutathione transferase

Keywords: mutagenesis مواجه با سایت; GSH; glutathione; GST; glutathione transferase; GSTO; Omega-class GST; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Crystal structure; Glutathione; Glutathione transferase; Lepidoptera; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Identification of important charged residues for alkali cation exchange or pH regulation of NhaH, a Na+/H+ antiporter of Halobacillus dabanensis

Keywords: mutagenesis مواجه با سایت; Halobacillus dabanensis; NhaH; Antiporter; Site-directed mutagenesis; Alkali cation translocation; pH regulation;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Exploration of the six tryptophan residues of Escherichia coli cystathionine β-lyase as probes of enzyme conformational change

Keywords: mutagenesis مواجه با سایت; Pyridoxal 5′-phosphate; Tryptophan fluorescence; Site-directed mutagenesis; Amino acid metabolism; Cystathionine;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Identification of the amino acid residues involved in the hemolytic activity of the Cucumaria echinata lectin CEL-III

Keywords: mutagenesis مواجه با سایت; CD; circular dichroism; CRD; carbohydrate-recognition domain; TBS; Tris-buffered saline; WT; wild-type; Lectin; Hemolysin; Pore-forming toxin; Oligomerization; Calcium ion; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Rational design and generation of recombinant control reagents for bispecific antibodies through CDR mutagenesis

Keywords: mutagenesis مواجه با سایت; Bispecific antibodies; Complementarity determining regions; Epidermal growth factor receptor; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Just an additional hydrogen bond can dramatically reduce the catalytic activity of Bacillus subtilis lipase A I12T mutant: An integration of computational modeling and experimental analysis

Keywords: mutagenesis مواجه با سایت; Bacillus subtilis lipase A; Hydrogen bond; Site-directed mutagenesis; Dynamics simulation; Quantum mechanics/molecular mechanics; Atom-in-molecules;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

A single amino acid determines the catalytic efficiency of two alkenal double bond reductases produced by the liverwort Plagiochasma appendiculatum

Keywords: mutagenesis مواجه با سایت; Alkenal double bond reductase; Hydroxycinnamyl aldehyde; Molecular modeling; Site-directed mutagenesis; Plagiochasma appendiculatum;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Computational design of short-chain dehydrogenase Gox2181 for altered coenzyme specificity

Keywords: mutagenesis مواجه با سایت; Computational design; Altered coenzyme specificity; Molecular dynamics simulation; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Improving the acidic stability of a β-mannanase from Bacillus subtilis by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; β-Mannanase; Site-directed mutagenesis; Acid stability; Bacillus subtilis;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Structural and functional consequences of EF-hand I recovery in mnemiopsin 2

Keywords: mutagenesis مواجه با سایت; Bioluminescence; Calcium; EF-hand loop; Mnemiopsin; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

A combination of site-directed mutagenesis and chemical modification to improve diastereopreference of Pseudomonas alcaligenes lipase

Keywords: mutagenesis مواجه با سایت; PAL; Pseudomonas alcaligenes lipase; DTNB; 5,5′-dithiobis-(2-nitrobenzoic acid); MALDI-TOF; matrix assisted laser desorption/ionization time of flight; WT; wide type; MD; molecular dynamic; Lipase; Diastereopreference; Site-directed mutagenesis; Chemica

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Binding mode characterization of 6α- and 6β-N-heterocyclic substituted naltrexamine derivatives via docking in opioid receptor crystal structures and site-directed mutagenesis studies: Application of the 'message-address' concept in development of mu op

Keywords: mutagenesis مواجه با سایت; Mu opioid receptor; Selective antagonists; Crystal structures; Automated docking; Site-directed mutagenesis; GPCR;

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions

Keywords: mutagenesis مواجه با سایت; OAS; O-acetylserine; AVG; aminoethoxyvinylglycine; CBL; cystathionine β-lyase; CGL; cystathionine γ-lyase; CGS; cystathionine γ-synthase; Cth; cystathionine; Hcys; homocysteine; HO-HxoDH; d-2-Hydroxyisocaproate dehydrogenase; IPTG; Isopropyl-β-d-thiog

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Characterization of the first eukaryotic cold-adapted patatin-like phospholipase from the psychrophilic Euplotes focardii: Identification of putative determinants of thermal-adaptation by comparison with the homologous protein from the mesophilic Euplotes

Keywords: mutagenesis مواجه با سایت; Patatin-like phosholipase; Molecular cold adaptation; Site-directed mutagenesis; Psychrophilic enzymes; PLP; patatin-like phospholipase; RATE; rapid amplification of telomeric ends; pNP; p-nitrophenyl; DiC3PC; 1,2-dipropinoyl-sn-glycero-3-phosphocholine;

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Introduction of a unique tryptophan residue into various positions of Bacillus licheniformis DnaK

Keywords: mutagenesis مواجه با سایت; DnaK; Bacillus licheniformis; Site-directed mutagenesis; Single tryptophan; Circular dichroism; Intrinsic tryptophan fluorescence;

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2013 سفارش ترجمه

Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis

Keywords: mutagenesis مواجه با سایت; Endoglucanase; pH-optimum; Cloning; Site-directed mutagenesis; CMC; carboxymethylcellulose; EG; endo-1,4-β-glucanase; PCR; polymerase chain reaction; Pv; Penicillium verruculosum; SDS-PAGE; sodium dodecylsulfate polyacrylamide gel electrophoresis; Tr; Tr

دانلود رایگان متن کامل مقاله ISI 29 صفحه سال انتشار : 2013 سفارش ترجمه

A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Separate PCR amplification; Homologous recombination; One-step ligation; Mutation frequency

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2013 سفارش ترجمه

Relationship between Escherichia coli AppA phytase's thermostability and salt bridges

Keywords: mutagenesis مواجه با سایت; Thermostability; Salt bridge; Molecular dynamics simulation; Escherichia coli AppA phytase; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2013 سفارش ترجمه

Mutational analysis of the oxygen-binding site of cholesterol oxidase and its impact on dye-mediated dehydrogenase activity

Keywords: mutagenesis مواجه با سایت; Cholesterol dehydrogenase; Cholesterol oxidase; FAD; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Analysis of amino acid residues involved in cold activity of monomeric isocitrate dehydrogenase from psychrophilic bacteria, Colwellia maris and Colwellia psychrerythraea

Keywords: mutagenesis مواجه با سایت; Isocitrate dehydrogenase; Cold-adapted enzyme; Site-directed mutagenesis; Colwellia maris; Colwellia psychrerythraea

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif

Keywords: mutagenesis مواجه با سایت; RNase(s) H, ribonuclease(s) H; Ec-RNase H1, E. coli RNase H1; Hs-RNase HC, the catalytic domain of human (Homo sapiens) RNase H1RNase H; Crystal structure; Active site motif; Conserved residue; C-Terminal tail; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

The role of Y84 on domain 1 and Y87 on domain 2 of Paragonimus westermani taurocyamine kinase: Insights on the substrate binding mechanism of a trematode phosphagen kinase

Keywords: mutagenesis مواجه با سایت; PK, phosphagen kinase; TK, taurocyamine kinase; AK, arginine kinase; CK, creatine kinase; GK, glycocyamine kinase; LK, lombricine kinasePhosphagen kinase; Taurocyamine kinase; Paragonimus westermani; Trematode; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2013 سفارش ترجمه

A double mutant of highly purified Geobacillus stearothermophilus lactate dehydrogenase recognises l-mandelic acid as a substrate

Keywords: mutagenesis مواجه با سایت; Geobacillus stearothermophilus lactate dehydrogenase (bsLDH); l-Mandelic acid; Site-directed mutagenesis; Substrate specificity; TAGZyme system

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Determination of binding points of methylene blue and cationic phenoxazine dyes on human butyrylcholinesterase

Keywords: mutagenesis مواجه با سایت; BChE inhibition; Site-directed mutagenesis; Molecular docking; Methylene blue; Meldola blue; Nile blue

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Changes on enantioselectivity of a genetically modified thermophilic lipase by site-directed oriented immobilization

Keywords: mutagenesis مواجه با سایت; Monocysteine mutants; Site-directed mutagenesis; Site-directed immobilization; Lipase; Enantioselectivity

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

A rational design for trypsin-resistant improvement of Armillariella tabescens β-mannanase MAN47 based on molecular structure evaluation

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Protease-resistance; Homology modeling; Overlap-extension PCR

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Directed evolution of a family 26 glycoside hydrolase: Endo-β-1, 4-mannanase from Pantoea agglomerans A021

Keywords: mutagenesis مواجه با سایت; β-1, 4-mannanase; DNA shuffling; Site-directed mutagenesis; Catalytic efficiency

دانلود رایگان متن کامل مقاله ISI 7 صفحه سال انتشار : 2013 سفارش ترجمه

Probing the molecular determinant of the lipase-specific foldase Lif26 for the interaction with its cognate Lip26

Keywords: mutagenesis مواجه با سایت; Acinetobacter sp.; Lipase; Lipase specific foldase; Protein interaction; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Structural and biochemical features of acidic α-amylase of Bacillus acidicola

Keywords: mutagenesis مواجه با سایت; Bacillus acidicola; α-Amylase; Circular dichroism; Site-directed mutagenesis; Fluorescence quenching;

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Structure-based engineering of histidine residues in the catalytic domain of α-amylase from Bacillus subtilis for improved protein stability and catalytic efficiency under acidic conditions

Keywords: mutagenesis مواجه با سایت; Bacillus subtilis α-amylase; Site-directed mutagenesis; Acidic stability; Structure model

دانلود رایگان متن کامل مقاله ISI 8 صفحه سال انتشار : 2013 سفارش ترجمه

Solution structure of a mutant of the triheme cytochrome PpcA from Geobacter sulfurreducens sheds light on the role of the conserved aromatic residue F15

Keywords: mutagenesis مواجه با سایت; PpcA, Geobacter sulfurreducens triheme cytochrome encoded by gene GSU0612; PpcAF15L, PpcA mutant with residue F15 replaced by leucine; rmsd, root-mean-square deviationGeobacter; Multiheme cytochromes; NMR; Structure–function; Site-directed mutagenesis

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Implications of active site orientation in myoglobin for direct electron transfer and electrocatalysis based on monolayer and multilayer covalent immobilization on gold electrodes

Keywords: mutagenesis مواجه با سایت; Site-directed mutagenesis; Cyclic voltammetry; Electrocatalysis; Chemically-modified electrodes

دانلود رایگان متن کامل مقاله ISI 9 صفحه سال انتشار : 2013 سفارش ترجمه

Stereochemical mechanism of two sabinene hydrate synthases forming antipodal monoterpenes in thyme (Thymus vulgaris)

Keywords: mutagenesis مواجه با سایت; Thymus vulgaris; Monoterpene synthase; Sabinene hydrate; Stereochemical mechanism; Site-directed mutagenesis; Active-site modeling

دانلود رایگان متن کامل مقاله ISI 10 صفحه سال انتشار : 2013 سفارش ترجمه

Sulfur-containing amino acids in 7TMRs: molecular gears for pharmacology and function

Keywords: mutagenesis مواجه با سایت; GPCR; crystal structure; site-directed mutagenesis; molecular modeling

دانلود رایگان متن کامل مقاله ISI 12 صفحه سال انتشار : 2013 سفارش ترجمه

Site directed mutagenesis of StSUT1 reveals target amino acids of regulation and stability

Keywords: mutagenesis مواجه با سایت; Regulation of sucrose transport; Homodimerization; Membrane microdomains; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 13 صفحه سال انتشار : 2013 سفارش ترجمه

The N550K/H Mutations in FGFR2 Confer Differential Resistance to PD173074, Dovitinib, and Ponatinib ATP-Competitive Inhibitors

Keywords: mutagenesis مواجه با سایت; EGFR; epidermal growth factor receptor; FGFR; fibroblast growth factor receptor; RTK; receptor tyrosine kinase; SDM; site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 17 صفحه سال انتشار : 2013 سفارش ترجمه

Assignment of the CO-sensitive carboxyl group in mitochondrial forms of cytochrome c oxidase using yeast mutants

Keywords: mutagenesis مواجه با سایت; BNC; binuclear centre; CcO; cytochrome c oxidase; DDM; n-dodecyl-β-D-maltoside; 6H-WT; yeast CcO containing a six-histidine tag on nuclear-encoded Cox13 subunit; 6H-E243DI or 6H-I67NI; the 6H-WT strain with an additional mutation of E243D or I67N in mtDN

دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2012 سفارش ترجمه

Bidirectional fluorescence properties of pyrene-based peroxisome proliferator-activated receptor (PPAR) α/δ dual agonist

Keywords: mutagenesis مواجه با سایت; Peroxisome proliferator-activated receptor; PPARδ; PPARα; Fluorescent PPARα/δ co-agonist; Site-directed mutagenesis;

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Prediction of substrate-binding site and elucidation of catalytic residue of a phytase from Bacillus sp.

Keywords: mutagenesis مواجه با سایت; Bacillus phytase; Site-directed mutagenesis; Phytate; Catalytic site

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Site-directed mutagenesis and over expression of aroG gene of Escherichia coli K-12

Keywords: mutagenesis مواجه با سایت; PEP; phosphoenolpyruvate; E4P; erythrose 4-phosphate; DAHP; 3-deoxy-d-arabino-heptulosonate-7-phosphate; PCR; polymerase chain reaction; SOE-PCR; splicing overlap extension polymerase chain reaction; LB; Luria Bertani; IPTG; isopropyl-β-d-thiogalactopyra

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Supporting role of lysine 13 and glutamate 16 in the acid–base mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiae

Keywords: mutagenesis مواجه با سایت; Lysine biosynthesis; Saccharopine dehydrogenase; Site-directed mutagenesis; Isotope effects; pH-rate profiles; Enzyme mechanism

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Improved reaction pattern of an endoglycanase from Paenibacillus cookii for chitosan oligosaccharide production

Keywords: mutagenesis مواجه با سایت; GHF, glycoside hydrolase family; PCR, polymerase chain reaction; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; TLC, thin layer chromatographyChitosanase; Chitosan oligosaccharide; Site-directed mutagenesis; Paenibacillus cookii

دانلود رایگان متن کامل مقاله ISI 5 صفحه سال انتشار : 2012 سفارش ترجمه

Increasing the storage and oxidation stabilities of N-acyl-d-amino acid amidohydrolase by site-directed mutagenesis of critical methionine residues

Keywords: mutagenesis مواجه با سایت; N-acyl-d-amino acid amidohydrolase; N-d-AAase; Variovorax paradoxus Iso1; Site-directed mutagenesis; Methionine oxidation; Enzyme stability;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

The β1 domain of protein G can replace the chorismate mutase domain of the T-protein

Keywords: mutagenesis مواجه با سایت; Monofunctional TyrA; Multidomain protein; Bifunctional protein; Site-directed mutagenesis; Protein–protein interaction

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Functional significance of some particular amino acid residues in Bombyx mori pyridoxal kinase

Keywords: mutagenesis مواجه با سایت; Bombyx mori; Pyridoxal kinase; Site-directed mutagenesis; Functional significance

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase

Keywords: mutagenesis مواجه با سایت; CBB; Coomassie Brilliant Blue R250; CDNB; 1-chloro-2,4-dinitrobenzene; ECA; ethacrynic acid; GTX; S-hexyl glutathione; GSH; glutathione; GST; glutathione transferase; 4-HNE; 4-hydroxynonenal; IPTG; isopropyl 1-thio-β-d-galactoside; LB; Luria-Bertani me

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Engineering a thermostable β-1,3-1,4-glucanase from Paecilomyces thermophila to improve catalytic efficiency at acidic pH

Keywords: mutagenesis مواجه با سایت; β-1,3-1,4-Glucanase; Directed evolution; Optimal pH; Site-directed mutagenesis; Paecilomyces thermophila

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel

Keywords: mutagenesis مواجه با سایت; Synthetic cephalosporin C acylase; 7-Aminocephalosporanic acid; Overexpression; Site-directed mutagenesis; Substrate transport tunnel

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه

A reporter system that discriminates EF-hand-sensor motifs from signal-modulators at the single-motif level

Keywords: mutagenesis مواجه با سایت; Monofunctional TyrA; Multidomain protein; Protein design; Site-directed mutagenesis; Calcium-sensor system;

دانلود رایگان متن کامل مقاله ISI 6 صفحه سال انتشار : 2012 سفارش ترجمه