Keywords: ترافیک; GHR; growth hormone receptor; GHRtm; recombinant fragment GHR254-294; JAK2; Janus kinase 2; RTK; receptor tyrosine kinase; TMD; transmembrane domain; ECD; extracellular domain; ICD; intracellular domain; JM; juxtamembrane; ecJM; extracellular juxtamembr
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Rapid NMR-scale purification of 15N,13C isotope-labeled recombinant human STIM1 coiled coil fragments
Keywords: ترافیک; Signal protein; Oligomerization; Calcium channel; Coiled coil; NMR spectroscopy; STIM1; stromal interaction molecule 1; SOCE; store operated calcium entry; ER; endoplasmic reticulum; PM; plasma membrane; CRAC; calcium release-activated channels; NMR; nucl
Solution NMR views of dynamical ordering of biomacromolecules
Keywords: ترافیک; ALS; Amyotrophic Lateral Sclerosis; CPMG; Carr-Purcell-Meiboom-Gill; CPP; Cell Penetrating Peptide; CHO; Chinese Hamster Ovary Cells; Rex; Chemical/Conformational Exchange; (C)EST; (Chemical) Exchange Saturation Transfer; CSA; Chemical Shift Anisotropy; C
Resolving biomolecular motion and interactions by R2 and R1Ï relaxation dispersion NMR
Keywords: ترافیک; CPMG; Carr-Purcell-Meiboom-Gill; EM; electron microscopy; NMR; nuclear magnetic resonance; NOE; nuclear Overhauser effect; TROSY; transverse relaxation-optimized spectroscopy; NMR spectroscopy; Protein dynamics; DNA/RNA dynamics; Chemical exchange;
Structure determination of supra-molecular assemblies by solid-state NMR: Practical considerations
Keywords: ترافیک; 1D, 2D, 3D, 4D; one-dimensional, two-dimensional, three-dimensional, four-dimensional; AM-XiX; Amplitude-Modulated XiX decoupling; AQ; acquisition time; BASE RFDR; BAnd SElective Radio Frequency Dipolar Recoupling; BMRB; Biological Magnetic Resonance Data
NMR Studies of Large Proteins
Keywords: ترافیک; large proteins; NMR spectroscopy; protein complexes; TROSY; transverse relaxation optimized spectroscopy; NOEs; nuclear Overhauser enhancements; PRE; paramagnetic relaxation enhancement; PhoA; alkaline phosphatase; TF; trigger factor;
NMR investigation of the isolated second voltage-sensing domain of human Nav1.4 channel
Keywords: ترافیک; CF; cell-free; DC7PC; 1,2-diheptanoyl-sn-glycero-3-phosphocholine; DDM; n-dodecyl β-d-maltoside; DHPC; 1,2-dihexanoyl-sn-glycero-3-phosphocholine; DMPC; 1,2-dimiristoyl-sn-glycero-3-phosphocholine; DMPG; 1,2-dimiristoyl-sn-glycero-3-phosphoglycerol; DOPG
Solution conformations of a linked construct of the Zika virus NS2B-NS3 protease
Keywords: ترافیک; Inhibitor; NMR spectroscopy; NS2B-NS3 protease; Pseudocontact shifts; Zika; bZiPro; Zika virus NS2B-NS3 protease expressed in two individual parts; eZiPro; Zika virus NS2B-NS3 protease expressed with an enzymatic cleavage site between NS2B and NS3pro; gZi
Applications of PLOR in labeling large RNAs at specific sites
Keywords: ترافیک; NMR; nuclear magnetic resonance; PLOR; position-selective labeling of RNA; T7 RNAP; T7 RNA polymerase; nt; nucleotides; smFRET; single molecule Förster resonance-energy transfer; SPE; solid-phase extraction; PCR; polymerase chain reaction; NOESY; nuclear
S-Nitrosylation Induces Structural and Dynamical Changes in a Rhodanese Family Protein
Keywords: ترافیک; NO; nitric oxide; WT; wild-type; SNOC; S-nitrosocysteine; TROSY; transverse relaxation optimized spectroscopy; MS; mass spectrometry; NOE; nuclear Overhauser enhancement; rhodanese; post-translational modification; nuclear magnetic resonance (NMR); X-ray
ARTSY-J: Convenient and precise measurement of 3JHNHα couplings in medium-size proteins from TROSY-HSQC spectra
Keywords: ترافیک; Backbone torsion angle; Gly; Karplus equation; TROSY; GB3; HIV-1 Protease; Protein NMR;
Effects of nucleotide binding to LmrA: A combined MAS-NMR and solution NMR study
Keywords: ترافیک; ABC; ATP binding cassette; ADP; adenosine diphosphate; ATP; adenosine triphosphate; CP; cross polarization; DDM; n-dodecyl-β-d-maltoside; EM; electron microscopy; EPR; electron paramagnetic resonance; HSQC; heteronuclear single quantum coherence; INEPT;
NMR conformational properties of an Anthrax Lethal Factor domain studied by multiple amino acid-selective labeling
Keywords: ترافیک; ALF; Anthrax Lethal Factor; TROSY; transverse relaxation optimized spectroscopy; IPTG; isopropyl β-d-1-thiogalactopyranoside; N-/C-ALF; N-/C-terminal domain of Anthrax Lethal Factor; Anthrax Lethal Factor; NMR spectroscopy; Selective labeling strategies;
NMR-based approach to measure the free energy of transmembrane helix-helix interactions
Keywords: ترافیک; NMR; nuclear magnetic resonance; DPC; dodecylphosphocholine; TM; transmembrane; TMD; transmembrane domain; LPR; lipid to protein ratio; CMC; critical micelle concentration; FRET; Forster resonance energy transfer; FGFR3; fibroblast growth factor receptor
Global fold and backbone dynamics of the hepatitis C virus E2 glycoprotein transmembrane domain determined by NMR
Keywords: ترافیک; 4-HTB; 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl benzoate; CPMG; Carr-Purcell-Meiboom-Gill; DSS; 4,4-dimethyl-4-silapentane-1-sulfonic acid; HCV; hepatitis C virus; hetNOE; hetero-nuclear {1H}-15N-nuclear Overhauser effect; HSQC; heteronuclear
Information-driven modeling of large macromolecular assemblies using NMR data
Keywords: ترافیک; Biomolecular complexes; Modeling; Docking; Integrative structural biology; TROSY; Methyl TROSY;
Indirect use of deuterium in solution NMR studies of protein structure and hydrogen bonding
Keywords: ترافیک; TROSY; transverse relaxation optimized spectroscopy; BIRD; bilinear rotation decoupling; CPMG; Carr-Purcell-Meiboom-Gill pulse train; QCC; quadrupolar coupling constant; CSA; chemical shift anisotropy; RDC; residual dipolar coupling; NOE; nuclear Ov
Contribution of the two domains of E. coli 5â²-nucleotidase to substrate specificity and catalysis
Keywords: ترافیک; 5NT; E. coli 5â²-nucleotidase; AMPCP; α,β-methylene-ADP; NDomN; terminal domain with residues 26-362 and a C-terminal hexahistidine tag; CDom; C-terminal domain with residues 363-550 and a C-terminal hexahistidine tag; pNPP; para-nitrophenyl phosph
Preferential domain orientation of HMGB2 determined by the weak intramolecular interactions mediated by the interdomain linker
Keywords: ترافیک; HSQC; heteronuclear single quantum coherence; TROSY; transverse relaxation optimized spectroscopy; NOE; nuclear Overhauser effect; IPAP-HSQC; in-phase and anti-phase HSQC; RDC; residual dipolar coupling; SAXS; small-angle X-ray scattering; DIORITE; determ
NMR studies of weak protein-protein interactions
Keywords: ترافیک; CPMG; Carl-Purcell-Meiboom-Gill; CSP; chemical shift perturbation; ETDA; ethylenediaminetetraacetic acid; HADDOCK; High Ambiguity Driven protein-protein DOCKing; nOe; nuclear Overhauser effect; HSQC; heteronuclear single-quantum coherence; IDIS-NM
The C-terminal domain of human Rev1 contains independent binding sites for DNA polymerase η and Rev7 subunit of polymerase ζ
Keywords: ترافیک; TLS; translesion synthesis; Rev1-CT; C-terminal domain of Rev1; RIR; Rev1 interacting region; NMR; nuclear magnetic resonance; TROSY; transverse relaxation optimized spectroscopy; ITC; isothermal titration calorimetry; aa; amino acid; DNA damage tolerance
The transmembrane domain of caveolin-1 exhibits a helix-break-helix structure
Keywords: ترافیک; LMPG; lyso-myristoylphosphatidylglycerol; NMR; nuclear magnetic resonance spectroscopy; CD; circular dichroism spectroscopy; CSI; chemical shift indexing; HSQC; heteronuclear single quantum coherence; TROSY; transverse relaxation optimized spectroscopy; C
Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes
Keywords: ترافیک; Brij-35; dodecylpolyoxyethylene 35; Brij-58; hexadecylpolyoxyethylene 58; Brij-78; octadecylpolyoxyethylene 78; Brij-98; octadecylpolyoxyethylene 98; CF; cell-free; DC7PC; 1,2-diheptanoyl-sn-glycero-3-phosphocholine; DHPC; 1,2-dihexanoyl-sn-glycero-3-phos
Hsp90 structure and function studied by NMR spectroscopy
Keywords: ترافیک; Protein folding; TROSY; Protein-protein interaction; Selective isotope labelling;
Paramagnetic tagging for protein structure and dynamics analysis
Keywords: ترافیک; CLaNP; caged lanthanide NMR probe; IPAP; in-phase anti-phase; PCS; pseudocontact shift; LBP; lanthanide binding peptide; NOE; nuclear Overhauser effect; PRE; paramagnetic relaxation enhancement; RDC; residual dipolar coupling; R1para; paramagnetically enh
Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements
Keywords: ترافیک; CAPRI; Critical Assessment of PRedicted Interactions; HSQC; heteronuclear single quantum correlation; RDC; residual dipolar coupling; TEV; tobacco etch virus; TROSY; transverse relaxation-optimized spectroscopy; Ub; ubiquitin; YUH; yeast ubiquitin hydrola
Dynamics of biomolecules from picoseconds to seconds at atomic resolution
Keywords: ترافیک; Protein; Dynamics; Spectral density; Labeling; Spin; Order parameter; Relaxation; RDC; Dispersion; Exchange; TROSY;
Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1–7 and LSm2–8 Rings
Keywords: ترافیک; snRNP, small nuclear ribonucleoprotein; snRNA, small nuclear RNA; NOESY, nuclear Overhauser enhancement spectroscopy; TROSY, transverse relaxation optimized spectroscopy; LSm, Like SmmRNA degradation; mRNA splicing; NMR spectroscopy; TROSY; macromolecular
Analysis of non-uniformly sampled spectra with multi-dimensional decomposition
Keywords: ترافیک; 3DD, 4DD; three or four-dimensional versions of MDD, respectively; Co-MDD; co-processing of several spectra by MDD; DFT; discrete Fourier transform; E.COSY; exclusive correlation spectroscopy; FID; free-induction decay, typically refers to 1D spectrum in
Guidelines for the use of band-selective radiofrequency pulses in hetero-nuclear NMR: Example of longitudinal-relaxation-enhanced BEST-type 1H-15N correlation experiments
Keywords: ترافیک; Fast NMR; Proteins; Nucleic acids; Selective pulses; Pulse shaping; Longitudinal relaxation enhancement; HSQC; TROSY;
Measurement and interpretation of 15N-1H residual dipolar couplings in larger proteins
Keywords: ترافیک; TROSY;
Chemical shift tensor - The heart of NMR: Insights into biological aspects of proteins
Keywords: ترافیک; CODEX; centerband-only detection of exchange; CPMAS; cross polarization magic angle spinning; CPMG; Carr-Purcell-Meiboom-Gill; CRAMPS; combined rotational and multipulse spectroscopy; CRINEPT; polarization transfer by cross-correlated relaxation app
Measurement of one and two bond N-C couplings in large proteins by TROSY-based J-modulation experiments
Keywords: ترافیک; RDC; Bicelle; Myristoylated protein; Scalar coupling; J-modulation; TROSY;
Stable isotope labeling methods for protein NMR spectroscopy
Keywords: ترافیک; Stable-isotope-aided NMR method; Protein structure; TROSY; SAIL; Segmental labeling;
TROSY-based NMR experiments for NMR studies of large biomolecules
Keywords: ترافیک; TROSY; NMR of large biomolecules; ZQ-TROSY; SQ-TROSY;
TROSY of side-chain amides in large proteins
Keywords: ترافیک; TROSY; NMR spectroscopy; Sensitivity enhancement; Isotopomer selectivity; Stereospecific NMR assignment; Side-chain amides;
A 'just-in-time' HN(CA)CO experiment for the backbone assignment of large proteins with high sensitivity
Keywords: ترافیک; HNCACO; TROSY; Backbone experiment; Just-in-time experiment;
Effective rotational correlation times of proteins from NMR relaxation interference
Keywords: ترافیک; NMR; Cross-correlated relaxation; TROSY; Brownian motion; Effective rotational correlation time;
Cogwheel phase cycling in common triple resonance NMR experiments for the liquid phase
Keywords: ترافیک; Cogwheel phase cycling; Triple resonance; Coherence selection; Accelerated acquisition; Protein structure determination; TROSY;
NMR of membrane proteins in solution
Keywords: ترافیک; Membrane protein; NMR; Structure; Dynamics; Function; TROSY; Residual dipolar coupling; Paramagnetic; Relaxation; Spin label; Glycophorin A; Diacylglycerol kinase; KcsA; OmpA; OmpX; PagP; Phospholamban; Mistic;
Interaction of the replication terminator protein of Bacillus subtilis with DNA probed by NMR spectroscopy
Keywords: ترافیک; RTP; Terminator DNA; Protein-DNA complex; NMR spectroscopy; TROSY; HSQC titration; Structural perturbation; Winged-helix protein; Duplex DNA;
Measuring 1H-1H and 1H-13C RDCs in methyl groups: example of pulse sequences with numerically optimized coherence transfer schemes
Keywords: ترافیک; Coherence and polarization transfer; Molecular dynamics; Simulated annealing; Optimization of NMR experiments; Methyl groups; TROSY;