NMR Studies of Large Proteins

- Recent developments have allowed NMR studies of large proteins.
- NMR can determine structures of dynamic protein complexes.
- Structures of molecular chaperones in complex with non-native proteins were solved by NMR.

Recent breakthroughs in isotope-labeling and pulse sequence techniques have enabled the NMR characterization of large protein systems with molecular masses of hundreds of kilodaltons. NMR studies of a great variety of large proteins have provided unique insights into the binding, dynamic, and allosteric mechanisms. Here we present a brief summary of these developments by highlighting few cases that exemplify the uniqueness of NMR in providing atomic resolution information into key dynamic processes and structures of protein complexes with high degree of flexibility.

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