Keywords: PEDV; Papain-like protease; 6-Thioguanine; Noncompetitive inhibition; Alpha coronavirus; AUC; The abbreviations used are analytical ultracentrifugation; CoV; coronavirus; DUB; deubiquitinating; MERS; Middle East respiratory syndrome; βME; β-mercaptoetha
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Keywords: βME; β-mercaptoethanol; BS3; bis[sulfosuccinimidyl]suberate; BSA; bovine serum albumin; CLP; coactosin-like protein; cv; column volume; DF; Dicer fragment containing amino acids 1650-1912; DPBS; Dulbecco's PBS; DSF; differential scanning fluorimetry;
Keywords: AMPPNP; adenylylimidodiphosphate; AUC; analytical ultracentrifugation; βME; β-mercaptoethanol; Chk2; checkpoint kinase 2; DAPK3; Death-associated protein kinase 3; DTT; dithiothreitol; FHA; forkhead-associated; HU; hydroxyurea; IPTG; Isopropyl β-d-1-th
Keywords: Protein translocase; Biological weapons; Botulism; Antibotulinal agents; Modular nanomachine; βME; beta-mercaptoethanol; ÎpH; pH gradient across the cis and trans compartments; γ; single channel conductance; BoNT; botulinum neurotoxin; BTD; beltless tr
Crystal structure of the Eg5 - K858 complex and implications for structure-based design of thiadiazole-containing inhibitors
Keywords: 1,3,4-thiadiazole; K858 enantiomers; Eg5; Antimitotic drugs; Eg5-K858 complex; Rational drug design; KSP; Kinesin spindle protein; MT; Microtubule; PDB; Protein Data Bank; PK; Pyruvate kinase; LDH; Lactate dehydrogenase; ITC; Isothermal titration calorime
Disulfiram can inhibit MERS and SARS coronavirus papain-like proteases via different modes
Keywords: MERS- and SARS-CoV; Papain-like protease; Disulfiram; 6-Thioguanine; Mycophenolic acid; Synergistic inhibition; CoV; coronavirus; Mpro; main protease; DDC; diethyldithiolcarbamate; DUB; deubiquitination; MERS; Middle East respiratory syndrome; βME; β-me
Functional and conformational transitions of mevalonate diphosphate decarboxylase from Bacopa monniera
Keywords: Mevalonate diphosphate decarboxylase; Unfolding; Aggregation; Fluorescence quenching; CD spectroscopy; BmMDD; Bacopa monniera mevalonate diphosphate decarboxylase; CD; circular dichroism; GdnHCl; Guanidine hydrochloride; MEP; 2-C methyl-d-erythritol-4-pho
Steady-state kinetic studies reveal that the anti-cancer target Ubiquitin-Specific Protease 17 (USP17) is a highly efficient deubiquitinating enzyme
Keywords: Ubiquitin Specific Protease 17; DUB3; Ubiquitin; Protein refolding; USP17; Deubiquitinating; DUB; deubiquitinating enzyme; USP; Ubiquitin Specific Protease; Ub; ubiquitin; CDC25A; cell division cycle 25A; RCE1; Ras converting enzyme 1; IPTG; isopropyl β-
Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo
Keywords: Neurons; Somatic cells; Astrocytes; Trans-differentiation; Defined medium; bFGF; basic fibroblast growth factor; βMe; β-mercaptoethanol; BM-hMSCs; mesenchymal stem cells from human bone marrow; EGF; epidermal growth factor; ESCs; embryonic stem cells; G
Redox regulation of Rac1 by thiol oxidation
Keywords: ABD-f; 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole; βME; β-mercaptoethanol; CD; circular dichroism; DMEM; Dulbecco's modified Eagle׳s medium; DTPA; diethylenetriaminepentaacetic acid; DTT; dithiothreitol; EDTA; ethylenediaminetetraacetic acid; eN
Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR
Keywords: AMPPNP; adenosine 5â²-(β,γ-imido)triphosphate; βME; 2-mercaptoethanol; CW; continuous wave; EGTA; ethylene glycol-bis(l-aminoethyl ether)-N,N,Nâ²,Nâ²-tetraacetic acid; EM; electron microscopy; MSL; 4-maleimido-2,2,6,6-tetramethyl-1-piperidinoxy; NN;
Aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone III, exhibits substrate control
Keywords: ACN; acetonitrile; AeaJHAMT; Aedes aegypti juvenile hormone acid methyl transferase; βME; β-mercaptoethanol; CA; corpora allata; CbSAMT; salicylic acid carboxyl methyltransferase from Clarkia breweri; EDTA; ethylenediaminetetraacetic acid; EST; expresse
Crystal structure of Src-like adaptor protein 2 reveals close association of SH3 and SH2 domains through β-sheet formation
Keywords: βME; β-mercaptoethanol; CrkL; Crk-like; DTT; dithiothreitol; Itk; interleukin-2 tyrosine kinase; SLAP; Src-like adaptor protein; SFK; Src family kinase; SH; Src-homology; SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel electrophoresis; TCR; T cell r
Molecular mechanism of oxidation-induced TDP-43 RRM1 aggregation and loss of function
Keywords: TDP-43; trans-activation region DNA-binding protein of 43-kDa; RRM; RNA recognition motif; ALS; amyotrophic lateral sclerosis; FTLD; frontotemporal lobar degeneration; AD; Alzheimer's disease; PD; Parkinson's disease; HD; Huntington's disease; NTD;
Redox metabolism in Trypanosoma cruzi: Functional characterization of tryparedoxins revisited
Keywords: Grx; glutaredoxin; GR; glutathione reductase; TR; trypanothione reductase; T(SH)2; trypanothione; TS2; trypanothione disulfide; GSSG; glutathione disulfide; GSH; reduced glutathione; TXNPx; 2-Cys-peroxiredoxin-type tryparedoxin peroxidase; GPxI; glutathio
Neriifolin S, a dimeric serine protease from Euphorbia neriifolia Linn.: Purification and biochemical characterisation
Keywords: BSA; bovine serum albumin; βme; β-mercaptoethanol; CBB; Coomassie brilliant blue; DFP; diisopropylfluorophosphate; DMSO; dimethylsulphoxide; DTNB; 5,5â²-dithiobis-(2-nitrobenzoic acid); DTT; dithiothreitol; EDTA; ethylene di amine tetra acetic acid; EG
High yield expression and purification of HIV-1 Tat1â72 for structural studies
Keywords: βME; β-mercaptoethanol; CDK9; cyclin-dependent kinase 9; DSS; 2,2-dimethyl-2-silapentane-5-sulfonate; DTT; dithiothreitol; HSQC; heteronuclear single quantum coherence; Hexim1; hexamethylene bisacetamide-inducible protein 1; HAD; HIV-associated dementia
Beta tubulin affects the aryl hydrocarbon receptor function via an Arnt-mediated mechanism
Keywords: AhR; aryl hydrocarbon receptor; Arnt; Ah receptor nuclear translocator; HIF-1α; hypoxia inducible factor 1 alpha; βME; β-mercaptoethanol; DRE; dioxin response element; HRE; hypoxia response element; βNF; β-napthoflavone; HEDG; 25 mM HEPES, pH 7.4, 1
Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii
Keywords: Euphorbia milii; Euphorbiaceae; Serine proteases; Plant endopeptidases; Milin; Anti-milin; NEM; N-ethyl maleimide; EDTA; ethylene diammine tetraacetic acid; EGTA; ethylene glycol-bis (α-amino ethyl ether); BAPA; NR-benzoylarginine p-nitroanilide; DFP; di
On the chaperonin activity of GroEL at heat-shock temperature
Keywords: ATP; adenosine triphosphate; βME; β-mercaptoethanol; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Th T; thioflavin T; GroEL complex; Rhodanese; Chaperone; Heat shock temperature; Stability; Refolding;
Disulfide-linked dimers of human adrenaline synthesizing enzyme PNMT are catalytically active
Keywords: AdoHcy; S-adenosyl-l-homocysteine; AdoMet; S-adenosyl-l-methionine; βME; β-mercaptoethanol; AUC; analytical ultracentrifugation; DTT; dithiothreitol; EDTA; ethylene-diamine tetraacetic acid; GSH; reduced glutathione; GSSG; oxidized glutathione; PAGE; po