کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10106948 | 1615014 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute sensitivity of astrocytes in the Substantia Nigra to oxygen and glucose deprivation (OGD) compared with hippocampal astrocytes in brain slices
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کلمات کلیدی
PBSOGDSubstantia nigra pars reticulataaCSFMAP2SNRSNCROIGFAP[K+]o - [K +] OElectrophysiology - الکتروفیزیولوژیLight transmittance - انتقال نورImmunocytochemistry - ایمونوسیتوشیمیParkinsońs disease - بیماری پارکینسونImaging - تصویربرداریsubstantia nigra pars compacta - توده سیاه پارس متراکمtyrosine hydroxylase - تیروزین هیدروکسیلازDirect Current - جریان مستقیمAnoxic depolarization - دپولاریزاسیون آنوکسیPhosphate buffered saline - فسفات بافر شورartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیOxygen glucose deprivation - محرومیت گلوکز اکسیژنregion of interest - منطقه مورد نظرRat - موش صحراییCell membrane potential - پتانسیل غشای سلولیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالMicrotubule associated protein 2 - پروتئین مرتبط با میکروتوبول 2Glia - یاختههای گلیال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The Substantia Nigra is a brainstem nucleus critical for movement control. Although its dopamine-producing neurons degenerate in Parkinsons disease, little is known of the acute effects of ischemia in this region. We recently reported that oxygen and glucose deprivation (OGD) in brain slices, an in vitro ischemia model, evokes a profound depolarization and swelling of GABAergic neurons in the Substantia Nigra pars reticulata (SNr), but not dopaminergic neurons in the Substantia Nigra pars compacta (SNc). The current study characterised the effects of OGD on nigral astrocytes, and compared these with the established responses of astrocytes in the CA1 hippocampal region. Intracellular recordings were made from astrocytes at the border between SNc and SNr subregions, in midbrain slices from postnatal day 21-23 rats. Immunoreactivity for astrocyte-specific proteins was also assessed. OGD evoked a slow, then fast depolarization of nigral astrocytes. The fast phase developed during the anoxic depolarization (indicated by a fast negative shift of extracellular DC potential and increase in light transmittance) and rapid increase in extracellular K+ concentration in the SNr. This biphasic response resembled the OGD-evoked depolarization of hippocampal astrocytes. However, unlike the partial repolarization seen in hippocampal cells after reperfusion with O2 and glucose, nigral astrocytes remained depolarized near 0â¯mV. In addition, immunoreactivity for glial fibrillary acidic protein-positive astrocytes markedly decreased in the Substantia Nigra after OGD, while in the hippocampus remained unchanged. These data indicate an acute post-ischemic withdrawal of astrocytic support in the Substantia Nigra, but not in the hippocampus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 685, 15 October 2018, Pages 137-143
Journal: Neuroscience Letters - Volume 685, 15 October 2018, Pages 137-143
نویسندگان
Rashika N. Karunasinghe, Justin M. Dean, Janusz Lipski,