کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10107642 | 1616796 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of environmental stresses on the activities of the uspA, grpE and rpoS promoters of Escherichia coli O157:H7
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Heat shock proteins and RNA polymerase sigma factor play an important role in protecting cells against environmental stresses, including starvation, osmotic and oxidative stresses, and cold shock. In this study, the effect of environmental stresses on activity of the auto-fluorescent Escherichia coli O157:H7 generated by the fusion of gfpuv to E. coli uspA, grpE and rpoS promoters were examined. Osmotic shock caused about a 4-fold increase in green fluorescence of E. coli O157:H7 harboring uspAâ·gfpuv or rpoSâ·gfpuv at 37 °C and room temperature whereas osmotic shock at 5 °C did not induce green fluorescence. When starved, E. coli O157:H7 possessing grpEâ·gfpuv was more sensitive for evaluating stress at low temperature while uspAâ·gfpuv was better suited for detecting the stress response at higher temperature. The uspA, grpE and rpoS promoters were up-regulated to varying degrees by stresses commonly encountered during food processing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 99, Issue 1, 1 March 2005, Pages 91-98
Journal: International Journal of Food Microbiology - Volume 99, Issue 1, 1 March 2005, Pages 91-98
نویسندگان
Purushottam V. Gawande, Mansel W. Griffiths,