Targeted lipidomics profiling of marine phospholipids from different resources by UPLC-Q-Exactive Orbitrap/MS approach

Marine phospholipids (MPLs) are valuable components that can be applied within diverse areas like nutrition, pharmacy, and medicine as well as basic scientific research, particularly due to their high concentration of long chain polyunsaturated fatty acids. In this work, lipidomics approach by UPLC-Q-Exactive Orbitrap/MS was used for the identification, quantification, comparison, and characterization of phospholipids (PLs) from three different marine resources (shrimp head, codfish roe, and squid gonad). In total, 310 PL molecular species containing 34 different structures of fatty acid chains were identified simultaneously by Lipidsearch software. Significant differences between three groups in the PL classes, compositions and contents were revealed. A list of characteristic PL species that represent significant differences among groups was determined by lipidomics analysis. Until now, the information about the deep comprehensive description of PL from marine sources is limited. Thus, this study will give a very potential starting point to develop MPL products and establish the quality standards for different marine raw materials.