کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10127898 1645111 2018 39 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid UHPLC-HILIC method for algal guanosine 5′-diphosphate 3′-diphosphate (ppGpp) and the potential separation mechanism
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A rapid UHPLC-HILIC method for algal guanosine 5′-diphosphate 3′-diphosphate (ppGpp) and the potential separation mechanism
چکیده انگلیسی
A fast and facile hydrophilic interaction liquid chromatography (HILIC) method was developed and applied to quantify physiologically important ppGpp and its analogues in a tough sample, the astaxanthin-accumulating alga Hameatococcus pluvialis. The method is able to analyze simultaneously seven nucleotides, including ppGpp at the order of pmol g−1 cells within 12 min. Mechanism on the elution order was investigated. It was found that 1) phosphate salt competed for the amide groups on the HILIC column with the phosphate groups of the nucleotides; 2) intramolecular hydrogen bonds might contribute to the elution order by offsetting and reducing the number of free hydrogen acceptor/donor of the nucleotide molecules interacting with the amide groups. This is the first HILIC method for ppGpp, which is feasible and applicable to a wide range of samples, especially tough samples, e.g., algae and plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1096, 1 October 2018, Pages 143-153
نویسندگان
, , , , ,