کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10127899 | 1645111 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid and sensitive liquid chromatography/tandem mass spectrometry assay for simultaneous quantitation of disopyramide and its major metabolite, mono-isopropyl-disopyramide, in rat plasma and its application to a pharmacokinetic study
ترجمه فارسی عنوان
یک کروماتوگرافی مایع کروماتوگرافی سریع / حساس / اسپکترومتری توده دو طرفه برای اندازه گیری همزمان دیسپیراماید و متابولیت اصلی آن، مونویزپروپیل دیسپیرامید در پلاسمای موش و کاربرد آن در مطالعه فارماکوکینتیک
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Disopyramide as an antiarrhythmic agent has been used for treating ventricular tachycardia and metabolized into its major metabolite, mono-isopropyl-disopyramide, by CYP3A4. We developed a novel, selective, highly sensitive, accurate, rapid method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of disopyramide and mono-isopropyl-disopyramide in rat plasma. This study is the first report for the assay validation using LC-MS/MS in biological fluids after simple protein-precipitation method. The most sensitive signals by multiple reaction monitoring (MRM) showed at m/z 340.2â¯ââ¯239.2 and 298.2â¯ââ¯239.2 with same fragment ion for disopyramide and mono-isopropyl-disopyramide, respectively. The lower limit of quantification (LLOQ) was determined at 2â¯ng/mL for both analytes and the linear concentration ranges were found to be 2-2000â¯ng/mL for disopyramide and 2-1000â¯ng/mL for mono-isopropyl-disopyramide. Finally, this assay was successfully applied to pharmacokinetic analysis of disopyramide and mono-isopropyl-disopyramide after oral and intravenous administration of disopyramide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1096, 1 October 2018, Pages 154-159
Journal: Journal of Chromatography B - Volume 1096, 1 October 2018, Pages 154-159
نویسندگان
Quynh Khoa Pham, Hyewon Seo, Sung-Hoon Ahn,