کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10130375 | 1645335 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biochemical characterization of peroxidases from the moss Dicranum scoparium
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کلمات کلیدی
ABTSDMBQMDAXTTIEFNBTSDSnitroblue tetrazolium chloride - تترازولیم کلرید nitroblueDesiccation tolerance - تحمل تخریبisoelectric focusing - تمرکز ذره ای الکتریکیsuperoxide radical - رادیکال سوپراکسیدSOD - سدsodium dodecyl sulfate - سدیم دودسیل سولفاتSuperoxide dismutase - سوکسوکس دیسموتازmalondialdehyde - مالون دی آلدهیدMosses - مورچه هاBryophytes - یخبندان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Mosses are a convenient model to study stress responses of plants because of their remarkable stress tolerance. Peroxidase (EC 1.11.1.7) activities were tested in three moss species, namely Dicranum scoparium, Hylocomium splendens and Pleurozium schreberi growing together in the same location in a boreal forest. Peroxidase activity in D. scoparium was twice as high as in other mosses. Total peroxidase activity in unstressed D. scoparium was constitutively high; furthermore, long-term desiccation caused a significant increase in activity after 48â¯h of drying. Interestingly, when thalli desiccated for a week were rapidly rehydrated, peroxidase activity initially declined and then increased after 2â¯h rehydration. Diverse anionic and cationic isoforms were detected by native isoelectric focusing and PAGE of both crude extracts and partially purified peroxidases. The ability of peroxidases from D. scoparium to produce superoxide radical (O2
- â) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride (NBT) staining; specific O2
- â producing isoforms were revealed using 2D electrophoresis. Given a quinone and chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (
- OH), and production was increased by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic
- OH production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress tolerance.
- â) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride (NBT) staining; specific O2
- â producing isoforms were revealed using 2D electrophoresis. Given a quinone and chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (
- OH), and production was increased by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic
- OH production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress tolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: South African Journal of Botany - Volume 119, November 2018, Pages 132-141
Journal: South African Journal of Botany - Volume 119, November 2018, Pages 132-141
نویسندگان
A.O. Onele, A. Chasov, L. Viktorova, R.P. Beckett, T. Trifonova, F. Minibayeva,